Please provide a detailed solution for the following question You have constructed a standard curve from BSA protein for a Bradford Assay. The curve has 0, 1, 2, 3, and 4 ug protein in 50 µl water. You have an unknown protein with a concentration somewhere between 1-5 mg/mL. What serial dilution series should you use to guarantee at least two dilutions of your unknown fall below 3 ug (linear portion) on the standard curve? For example, would 1/2 x 1/2 x 1/2 (3 2-fold dilutions) be sufficiently dilute that two of the dilutions would give values within the linear portion of the standard curve?

Please provide a detailed solution for the following question You have constructed a standard curve from BSA protein for a Bradford Assay. The curve has 0, 1, 2, 3, and 4 ug protein in 50 µl water. You have an unknown protein with a concentration somewhere between 1-5 mg/mL. What serial dilution series should you use to guarantee at least two dilutions of your unknown fall below 3 ug (linear portion) on the standard curve? For example, would 1/2 x 1/2 x 1/2 (3 2-fold dilutions) be sufficiently dilute that two of the dilutions would give values within the linear portion of the standard curve?

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter14: Chromatography

Section: Chapter Questions

Problem 1P

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