POST-LAB QUESTIONS D. Barfoed's Test 1. Describe the evidence for a positive test. 2. What does a positive test indicate?_ 3. What is the chemical basis for a positive test? 4. Circle the names of the substances that gave a positive test. Glucose Fructose Lactose Sucrose Starch
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- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish ’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Seliwanoff’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.BARFOED’S TEST FOR MONOSACCHARIDESPlace 1 mL (20 drops) of each 1% carbohydrates solution in separate test tubes. Prepare a control with 1 mL water. Add 4 mL Barfoed’s reagent. Place the test tubes in boiling water bath for 10 minutes. Remove the test tubes and cool in cold water. Record your observations. A precipitate of cuprous oxide which may be green to red, indicates that a monosaccharide is present. Record your result and conclusion. Identify the compounds that are monosaccharides. SELIWANOFF’S TEST FOR KETOHEXOSESPlace 5 drops of 2% carbohydrate solution in separate test tubes. Prepare a control with 5 drops water. Add 1 mL of Seliwanoff’s reagent to each test tubes and heat. Record your results. The rapid formation of a bright red indicates that a ketohexose is present. Identify the compounds that are ketohexoses. IODINE TESTS FOR POLYSACCHARIDESPlace 2 mL of each carbohydrate solution in separate test tubes. Prepare a control with 2 mL of water. Add 1 drop of iodine solution…
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Barfoed’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)Complete the table below. Indicate what is observed if each test is positive. State what this result implies about each of the carbohydrates tested. TEST EXPECTED OBSERVATION FOR A POSITIVE TEST SUBSTANCE INDICATED BY A POSITIVE TEST I2 Solution Molisch Barfoed Seliwanoff Bial- OrcinolSuggest how the weak points of the Molisch test for carbohydrates can be strengthened by comparing it to other carbohydrate tests
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Benedict's test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.A deficiency in vitamin A can result in ________. improper bone development scurvy improper eye development or sight all of the aboveLipids in the diet can be ________. broken down into energy for the body stored as triglycerides for later use converted into acetyl CoA all of the above
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question)Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Fehling’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, video is very brief)Food Sample 2 (Unknown) Benedict's test Green precipitate A. reducing sugars not presnt B. Reducing sugar presennt Seliwanoff's Test Colorless solution A. Aldose present B. Ketose present C. Neither aldose or ketose present Iodine Test Colorless to brown solution A. Amlyose present B. Amlyose not present Give a summary of your results for Food Sample 2 (Unknown). What kind of food item do you think this might be? Consider the appearance of the sample and your test results for the sample.