Prepare a 1:200 dilution of the 20 mM Coomassie solution to measure the max wavelength—you need 2 ml for the absorbance measurement but the dilution does not matter as long as the absorbance is in the range of 0.2 to 0.6. Set the initial wavelength at 450 nm. Blank the spec with a tube containing 2 ml water. Use the 1000 ul pipette for water because you need to deliver the same amount of water in each tube for the next exercise.   Why is it when the calculations are done for the given values above, the value for ml is 0.01 for coomassie blue?

Question
Prepare a 1:200 dilution of the 20 mM Coomassie solution to measure the
max wavelength—you need 2 ml for the absorbance measurement but the dilution does not
matter as long as the absorbance is in the range of 0.2 to 0.6. Set the initial wavelength at 450
nm. Blank the spec with a tube containing 2 ml water. Use the 1000 ul pipette for water because
you need to deliver the same amount of water in each tube for the next exercise.
 
Why is it when the calculations are done for the given values above, the value for ml is 0.01 for coomassie blue?

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