prepare an assignment on Application of HPLC in Drug Analysis(Amiloride): one page show name of the drug, dosage form, extraction step, type of column, separation parameters e.g., flow rate, mobile phase, detection wavelength and run time
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- kindly prepare an assignment on Application of HPLC in Drug Analysis: one page show name of the drug, dosage form, extraction step, type of column, separation parameters e.g., flow rate, mobile phase, detection wavelength and run time. Submit before 05.11.Smaller particles affect HPLC column efficiency in what ways? A)increase the mass transfer term. B)diminish the pressure constraints of the mobile phase. C)reduce stationary phase retention D)enable lower slopes inthe C term E)cause higher temperature as a result of increased frictional heating.Column length: 25.0 cm Column diameter: 0.46 cm Stationary phase: C-18 Mobile phase: 80% methanol in water Flow rate: 1.0 mL/min Dead time: 5 min Peak width at base, WA = 20 Peak width at base, WB = 24
- Chemistry Column 25 m length and 0.2 mm I.D. has efficiency is 100,000 theoretical plates; Flow rate is 1 mL/min. Calculate the peak width for component with retention factor 2. Which data acquisition sampling rate should be used for proper quantification of this component? Explain. (For accurate integration peak should have at leastFT-IR technique can be utilized for the analysis of unknown analytes by matching it with__________. Both choices are correct Commercial library/database Reference standardWhat effect will the following have on plate height (H). Make sure to explain your full and complete reasoning. Increasing stationary phase thickness. Reducing the rate of sample injection. Increasing mobile phase flow rate. Decreasing temperature. Reducing stationary phase particle size.
- No need guideline answer if u provide guideline answer I will dislike ok Module Code & Title: CHM9011M Chromatography and Sep Sciences Description of Assessment Task and Purpose: To prepare an SOP for the GC-MS InstrumentIf some of the analytes were found to be out of range after doing the Quality Control and the machine repeatedly gives invalid result. What are the possible problem and best solution as a Medical Technologist?How the 2D Thin layer Chromatography separation processes are worked? Please shortly wtite at your own words. Answer should be to the point (5-6 lines maximum).
- Q) water hardness of each trial and average ppm with calculation, please. Hard Water Trial 1 Hard Water Trial 2 Hard Water Trial 3 Initial Syringe Reading 1.0ml 1.0ml 1.0ml Final Syringe Reading 0.88ml 0.84ml 0.85ml Volume of EDTA Consumed 0.12ml 0.16ml 0.15ml Water Hardness ppm CaCO3 Average ppmYou prepare the following MeOH standards from a 2000 ppm stock MeOH solution:Calibration Blank, 50 ppm, 100 ppm, 150 ppm and 400 ppm and analyze them using the GC-FID. You received the following data: Concentration of MeOH(ppm) Response (area) of MeOH peak (pA) 0 0 50 150 100 320 150 440 400 1100 Using the data above, make a properly documented graph of "Response (area)”versus "Concentration of MeOH (ppm)" and determine the linear workingrange (in units of concentration) of MeOH. This graph is to be done usingExcel. Be sure to provide a trendline, formula & R2 value).Example: A GC-FID analysis was conducted on a soil sample containing pollutant X. The following separations were conducted: t (minutes) peak area Injection 1 21.1 ppm Toluene internal Standard 10.11 36,242 33.4 ppm 14.82 45,997 Injection 2 21.1 ppm Toluene Internal Standard 10.05 38,774 unknown concentration X 14.77 39,115 What is the concentration of X in the sample?