Asked Jan 8, 2020

Protein X binds to Ligand W with a dissociation constant of .15 μmol. what is the concentration of W 75% of the protein is free (ie, unbound)?"


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Step 1

The concentration of ligand “W” is calculated in accordance with Hill-Langmuir, shown in equation (1), in which Kd, [W], and θ will be the dissociation constant, ligand concentration, and fraction of bounded protein respectively.

Chemistry homework question answer, step 1, image 1
Step 2

According to the given data, only 75% ligand is free so, the fraction of bounded pr...

Chemistry homework question answer, step 2, image 1

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