Provide one plausible explanation that would account for what may have gone wrong resulting in the described observations in each of the scenarios below. Scenario 1: All four plates (LB-, LB+, LBamp-, LBamp+) have growth on them when doing the Transformation woth apicilline
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Provide one plausible explanation that would account for what may have gone wrong resulting in the described observations in each of the scenarios below.
Scenario 1: All four plates (LB-, LB+, LBamp-, LBamp+) have growth on them when doing the Transformation woth apicilline
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- Provide one plausible explanation that would account for what may have gone wrong resulting in the described observations in each of the scenarios below Scenario 3: All four plates (LB-, LB+, LBamp-, LBamp+) have no growth on them when doing the Transformation experiment with Ampicilline.Suppose that you are given a short fragment of DNA to sequence. You amplify the fragment with PCR and set up a series of four dideoxy reactions. You then separate the products of the reactions by gel electrophoresis and obtain the following banding pattern:The following DNAs were incubated with E. coli DNA Polymerase I and dNTPs. Write the products expected in each reaction. Indicate the activity used in each reaction.
- Could you explain how you would know if you successfully induced transformation? Thank you Did you successfully demonstrate transformation occurred? Why or why not? (How do I know, what do I look for?) Explain the control used in this experiment. What was it, and why was it necessary? Based on the calculation in the question above, comment on the relative difficulty of achieving successful transformation of E. coli. (Please explain the relative difficulty)Compare and contrast ferric iron reduction withreductive dechlorination in terms of (1) product of thereduction and (2) environmental significance.polymerase chain reaction is a technology that requires high temperatures to reproduce DNA fragments. Explain why the discovery of thermophilic archaea and their associated DNA polymerase was critical to the success of this technique
- In the Messelsohn-Stahl experiment, there should be ______ band(s) in the centrifugation tube after two generations of bacterial growth in normal nitrogen. Group of answer choices None of these One light and one medium density Two light and heavy Three light bands One heavyRestriction enzymes are used in laboratory manipulations to cut DNA; many of these enzymes can be inactivated by incubating them at 80°C for 30 minutes. Why would this incubation inactivate the enzyme? Why do enzymes not simply operate better at higher temperatures, as other catalysts do?Knowing that the first allele of a studied gene has 763 pairs of bases, and the second one has 1232 pairs of bases, which one of those two molecules should migrate the furthest on the agarose gel once in the presence of an electric field? Justify your answer.
- Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?In the Avery experiment, mice were killed if they injected with S strain cells. Furthermore, incubation with extract from dead S strain cells could transform non pathogenic cells into pathogenic cells. This effect of S strain extract was eliminated if S strain extract is treated with what enzyme before incubation with non-pathogenic cells? protease DNAse RNAse Lipase