Provided in the Table below kinetic data for an enzymatic reaction that was carried out in the presence (last two columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in each reaction at a concentration of 2 mM. The enzyme concentration was similar in all and was approximately 0.001 иМ, a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve. b. Provide the type of inhibition for both? (1) V° [(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)] (2) V° (3) V° [S] (mM) 7.6 4.4 6.6 4 14.6 8.6 11.4 8. 26.6 16.4 17.8 16 45.8 29.8 24.6 24 60 40.8 28.2

Provided in the Table below kinetic data for an enzymatic reaction that was carried out in the presence (last two columns) and absence (second column = control) of enzyme inhibitor. Both inhibitors were added in each reaction at a concentration of 2 mM. The enzyme concentration was similar in all and was approximately 0.001 иМ, a. Calculate both Vmax and KM for the control using Lineweaver-Burk curve. b. Provide the type of inhibition for both? (1) V° [(umol/(ml.s)][(umol/(ml.s)] | [(umol/(ml.s)] (2) V° (3) V° [S] (mM) 7.6 4.4 6.6 4 14.6 8.6 11.4 8. 26.6 16.4 17.8 16 45.8 29.8 24.6 24 60 40.8 28.2

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter14: Chromatography

Section: Chapter Questions

Problem 9P

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