Purification table formula: A=Elc Absorption is 1.471 E= 27,000 (GFP) l= 1 cm 1.417=27,000 M^-1 (1 cm) (c) Given 5.44810^-5 M is the concentration convert the concentration units Molarity (mol/l) to mg molar mass of GFP is 27,000 g

Purification table formula: A=Elc Absorption is 1.471 E= 27,000 (GFP) l= 1 cm 1.417=27,000 M^-1 (1 cm) (c) Given 5.44810^-5 M is the concentration convert the concentration units Molarity (mol/l) to mg molar mass of GFP is 27,000 g

Introductory Chemistry For Today

8th Edition

ISBN:9781285644561

Author:Seager

Publisher:Seager

Chapter7: Solutions And Colloids

Section: Chapter Questions

Problem 7.37E

See similar textbooks

Related questions

Q: absorbance 0.8 0.7 y 1.4x- 0.01. 0.6 0.5 0.4 0.3 0.2 0.1 0.1 0.2 0.3 0.4 0.5 0.6 Concentration…

A: In the absorbance vs concentration graph : x-axis represents the concentration of sample. y-axis…

Q: Standardization of 1N Potassium Hydroxide Accurately weigh about 5 g of potassium biphthalate,…

A: Given that - Mass of potassium biphthalate = 5.1037 g Volume of Solution used = 75 mL Volume of…

Q: ОН N Ni2+ + 2 Ni + 2H+ ОН DMG FM 116.12 Bis(dimethylglyoximate)nickel(II) FM 288.91 FM 58.69 If the…

A: Amount of Ni present in the steel = (3/100) x 10 = 0.3 g Moles of Ni present = 0.3 / molar mass of…

Q: 8. What is the mass percent mim OF Kg COz (m/m) Solution Containing 260g K2C03 ana 1S49 for a H20 ?…

Q: The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the…

A: Given that: the initial volume of sample = 100.0 μL diluted volume = 2 mL +100.0 μL concentration…

Q: Part C The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer,…

A: Answer :- Part A :- M1V1 = 0.0514 (mol) Part B :- V1 = 6.42 (mL) Part C :- Original Concentration Of…

Q: A Cu2+ solution obtained by dissolving a 3.1871-g penny in 500 mL of nitric acid has an absorbance…

A: Given : Mass of penny = 3.1871 g Volume of solution = 500 mL = 0.500 L…

Q: For questions 48-55, please refer to the following: In order to form Ba(IO3)2, 500 mL of 0.5000…

A: Molarity of Ba(IO3)2 = 0.5 M Volume of Ba(IO3)2 = 500 ml Molarity of NaIO3 = 0.5 M Volume of NaIO3 =…

Q: Classify whether the following is an Instrumental error, Method error, Personal error, Indeterminate…

A: Since you have asked a question with multiple sub-parts, as per our company guidelines we are…

Q: A student creates a calibration plot for serial dilutions of Cu(NO3)2. A graph of concentration in M…

A: (a) y = 0.345x -0.11 Absorbance = y = 0.655 0.655 = 0.345x -0.11 x = 2.22 M (b) Molarity = Mass %…

Q: 4. You are placing the following quantities of BSA (1 µg/µL) in wells A1-A8 for a total volume of…

A: A table of data with mass and volume of BSA. Total volume and corresponding Absorbance values.

Q: A patient weighs 170 lb and is receiving a medication via an IV (600 mg/500 mL) infusion at 20 mL/hr…

A: Given : mass of patient = 170 lb Since 1 Kg = 2.205 lb Hence mass of patient in Kg = mass in lb /…

Q: 0.13 absorbance/4713.6 L/mol = ? Answer In scientific notation please.

Q: Which of the following variables does absorbance not depend upon? A. Molar absorptivity of the…

Q: In lab this week, assume you use a 25.00-ml volumetric pipet to transfer the standard red dye…

A: Volume of stock solution taken (V1) = 25 mL Volume of the solution made in volumetric flask (V2) =…

Q: The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the…

A: The volume of the diluted solution in L is; 1μL=1×10-6 L1 mL=1×10-3 LVt=2.00 mL+100.0 μL=2.00×10-3…

Q: Categorize each as a determinate or an indeterminate error and further classify determinate errors…

A: We have find out the types of errors. Note -as per our company guidelines we are supposed to answer…

Q: Given the following standard curve, answer the questions. See attachment A) If an unknown solution…

A: The graph reading should be done by sketching the line with given coordinate on X axis and Y axis…

Q: 24) Order: Potassium Chloride 75meq p.o. daily Supply: potassium Chloride 15 meq per 10 mL 220 mL…

A: We have find out dosage in fl-oz.

Q: After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40 and…

Q: The color change of a chemical requires an overtitration of 0.04 mL. Calculate the percent relative…

A: Given, Experimental value = 50 mL + 0.04 mL = 50.04 mL True value = 50 mL Calculating the percent…

Q: 2. Use the trendline equation from the calibration plot (that you wrote down in Table 2) to…

A: Solution: In colorimetry experiment we plot absorbance vs Concentration. we get a straight which…

Q: To determine the phosphate concentration in a water sample, the following standard curve was…

A: The calibration curve between the absorbance and concentration of phosphate ion is given as, Given:…

Q: 8. A student made a series of measurements of Mg** concentration in drinking water giving the…

A: Introduction : Drinking water contains magnesium high concentration of magnesium and sulfur is…

Q: ACCident Or Murder? You are a forensic scientist. You are investigating a murder involving poison.…

A: C6H5CH3 is actually methylbenzene. Mass of benzene used to prepare methyl benzene as told by the…

Q: The nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and…

A: The calibration curve equation given is, => y = 0.0699 x + 0.015 Where y = absorbance And x =…

Q: Construct a calibration curve based on the following data: Concentration Fe 0.100 0.500 2.00 4.00…

A: The beer’s law explain that the concentration of a solution is directly proportional to the…

Q: 1. A student performed this experiment and obtained the following concentration values: 0.02813 M,…

A: As per our guidelines we can only solve first question. Please resubmit other questions as next…

Q: For Question 2 and 3 please refer to the following diagram. The following data and diagram represent…

A: The relation of concentration and absorbance can be find with the help of graph between them. Here…

Q: In a laboratory 100 mL of phosphorus solution with concentration 1,0 mg/L is needed when the…

A: On dilution concentration changes but no of moles remain same. So, M1V1 = M2V2 M1 = Concentration of…

Q: A dose of 5 milligrams of penicillin is administered to a patient. The absorption constant for…

A: The formula given to be used is C = C0e-kt where C0 = initial concentration k = absorption…

Q: Caffeine (C8H10O2N4 • H2O) has an absorbance of 0.510 at 272 nm and 1 cm optical path in 1 mg / 100…

Q: The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the…

A: Given that: initial volume = 100.0 μL dilution volume = 2.00 mL + 100.0 μL concentration of…

Q: 4) But the real angler continues, regardless of the local sage. What sort of tone does the author…

A: The sort of tone does the author create by using the connotative effects of the word sage in…

Q: A sample was analysed for its Calcium concentration. A number of standard solutions were prepared…

A: The plot of concentration will give the relation between concentration and absorbance. If one…

Q: Explain the use of plans and their importance in assessing the quality of pharmaceutical products.…

A: A quality plan is document that outline core quality objectives to enhance the quality in the…

Q: A sample was prepared by diluting 1 mL of a sample to 5 mL. The resulting solution has an…

A: Beer's Law plot-

Q: The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the…

A: The volume of concentrated solution is 100.0µL Concentration of the diluted solution = 6.21×10-6 M…

Q: After determining the absorbance of several standards of known concentration, the trendline for a…

A: The equation for a straight line is given by y=mx+c where m is the slope and c is the y-intercept.…

Q: An 8 mL volumetric pipet is used to collect a sample from a 0.2015 M solution of NaOH. a. Estimate…

A: Given the molarity of NaOH solution = 0.2015 M Volume of the volumetric pipette used to collect NaOH…

Q: 9. The concentration of a solution (in ppb) was measured several times, resulting in the following…

A: Given : Concentration in ppb 7.45 8.49 6.91 7.32 The Accepted value for the…

Q: Convert your average ppm concentration (and standard deviation) of Ca from the ISE measurements…

A: "Since there are multiple sub-parts in this question and it is not mentioned that which one has to…

Q: Part 2 - Dilution series Concentration (M) Absorbance 30 55 75 85 92 0.100 0.0500 0.0250 0.0125…

A: Given

Q: Active Dosage Form Packaging Raw Materials Actual Yield Formulation Ingredient 1.75kgs calcium 160mg…

A: Note: Here the reactants are CaO and H2O. Hence, product can formed is Calcium hydroxide (Ca(OH)2)…

Q: I'm stuck on this one. =( Using the Beer Law to calculate this solution. A standard solution has a…

A: Given the concentration of standard solution, C = 6 g/dL Absorbance of the standard solution, A =…

Q: Calculate the concentration in ppm of Cd in a 2.00 mL sample which has been shown to contain 16.4 μg…

A: Given :- mass of Cd in sample = 16.4 μg Volume of sample = 2.00 mL…

Question

Purification table

formula: A=Elc

Absorption is 1.471

E= 27,000 (GFP)

l= 1 cm

1.417=27,000 M^-1 (1 cm) (c)

Given 5.44810^-5 M is the concentration

convert the concentration units Molarity (mol/l) to mg

molar mass of GFP is 27,000 g

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

Patients undergoing an upper gastrointestinal tract laboratory test are typically given an X-ray contrast agent that aids with the radiologic imaging of the anatomy. One such contrast agent is sodium diatrizoate, a nonvolatile water-soluble compound. A 0.378-m solution is prepared by dissolving 38.4 g sodium diatrizoate (NaDTZ) in.l.60 102 mL water at 3 1.2C (the density of water at 31.2C is 0.995 g/cm3). What is the molar mass of sodium diatrizoate? What is the vapor pressure of this solution if the vapor pressure of pure water at 31.2C is 34.1 torr?
100 cm3 0.1 M NaH2PO4 solution is mixed with 150 cm3 0.2 M H3PO4 solution. What are the totalconcentrations of Na+, H+ and PO3−4in the mixed solution?
What volume (in cm3) of 30 mass/mass%, ammonium solution is needed (with a density of0.892 g/cm3 ) to produce 1.0 dm3 of pH 11.0 solution?Kb = 1.8 x 10-5
A 25mL volumetric pipet is used to deliver a sample of the stock solution marked “0.6000M X2SO4” into a 100mL volumetric flask. Distilled water is added to the flask until it is about 3/4thfull, the solution is mixed well, then more water is added to fill it up to the calibration mark, then it is mixed again. Calculate the concentration of X+ion in the dilute solution made above.
When a Vitamin C (ascorbic acid; MM = 176.12 g mol-1) tablet is crushed, dissolved and titrated with 0.0340 M KIO3(aq) to a purple/blue endpoint (given by a starch indicator), the volume of KIO3 used is 29.80 mL. If 60 mg of ascorbic acid is the recommended dietary allowance (i.e., 100% of the RDA), then what is the % RDA for the Vitamin C in the tablet? KIO3(aq) + 5 KI + 6 H+ → 3 I2(aq) + 3 H2O I2 (aq) + ascorbic acid → 2 I- + dehydroascorbic acid
If the concentration of TCE at standard conditions (0oC and 1atm) is 15 μg/m3 , what is the corresponding concentration in ppmv (by volume)?
What is the primary standards and its amounts, and indicators used for the Volumetric Solution (VS): 1N Sulfuric acid? And why?
Calculate [ I- ] given the following information: In a test tube, 2.0 mL 0.060 M KI solution 4.0 mL Distilled H2O 2.0 mL 2.0 mM Na2S2O3 solution 2.0 mL 0.040 M H2O2 solution Total solution volume: 10 mL
1. 365 ppb of Zinc are found in a doll. What is it’s %(w/w)? 2. A toy was treated that resulted to 500mL solution. What’s the concentration of Lead if 25 micrograms of it was found? State the anser in ppm. 3. A 250mL stock solution of 1.5 M Arsenic was prepared. What is the required volume of the solution if you’re using it to prepare 50mL of 0.75 M Arsenic solution? 4. If the treatment used HNO3 as the solvent, what is the concentration of Cadmium in molality if the amount of the element found in a 250mL solution was 24mg? (The density of Cadmium is 8.7 g/mL.)
Volume of an unknown used was 30 mL, Initial Buret volume was 0 and the Final Buret volume was 8.5 mL. What is the molarity of the unknown solution if the Net volume of NaOH being used was 8.5 mL and Millimoles (mmoles) NaOH reacted was 0.791? Then, what is the Mass (g) of Acetic Acid in unknown sample and thr average percent (%) Acetic Acid? (assume density = 1g/mL)
The dictionary defines homogeneousas “uniform in compo-sition throughout.” River water is a mixture of dissolved com-pounds, such as calcium bicarbonate, and suspended soil particles. Is river water homogeneous? Explain
16.300g of calcium chloride are dissolved in 100.0mL of water. The final solution has avolume of 102.3mL. The density of pure water is 0.99707g/mL. [Calculate the density of thesolution.]