Q is an analog of substrate A that binds to enzyme X and produces the following kinetics: [A] V0 (µmole/ml/min) [Q] = 0 [Q] = 0.5 µM [Q] = 2 µM 1 µM 10 7 4 3 µM 20 16 10 10 µM 35 31 23 30 µM 43 41 36 80 µM 47 46 43 a) Plot the data in Lineweaver Burk plot form (Hint: there should be three plots of data on your graph) and determine the following: the KM and Vmax of the enzyme X in the absence of Q, and the KMapp the Vmaxapp at each concentration of Q. b) What type of inhibition is this? c) Calculate the inhibition constant (Ki) for Compound Q
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Q is an analog of substrate A that binds to enzyme X and produces the following kinetics:
[A] V0 (µmole/ml/min)
[Q] = 0 [Q] = 0.5 µM [Q] = 2 µM
1 µM 10 7 4
3 µM 20 16 10
10 µM 35 31 23
30 µM 43 41 36
80 µM 47 46 43
a) Plot the data in Lineweaver Burk plot form (Hint: there should be three plots of data
on your graph) and determine the following: the KM and Vmax of the enzyme X in the
absence of Q, and the KMapp the Vmaxapp at each concentration of Q.
b) What type of inhibition is this?
c) Calculate the inhibition constant (Ki) for Compound Q
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