
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Transcribed Image Text:Question 19
The polymerase chain reaction requires the following except
A primers complementary to the ends of the sequence to be amplified
B) carefully controlled temperature conditions
cycles of denaturation, annealing and extension
RNA polymerase to synthesize RNA primers
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- QUESTION 21 Observation of purified DNA as "beads on a string" first indicated the presence of: a. supercoiling b. double helix C. nucleosomes O d. base pairingarrow_forwardQUESTION 14 The Hershey and Chase experiments, in which radioactive phosphorus (32P) and radioactive sulfur (35S) were used, demonstrated that O a. DNA labeled with 32P is transferred from the bacteriophage to the virus O b. DNA may be the hereditary material, although bacteriophages transfer both DNA and proteins into their hosts O C. proteins labeled with 35S become deactivated and unable to be transferred O d. DNA labeled with 35S and proteins labeled with 32P can be traced over the course of an experiment O e. bacteriophages transfer their DNA, not their coat proteins, into their hosts QUESTION 15 Which of the following makes its own food using energy and simple raw materials it obtains from nonbiological sources? O a. Cell O b. Producer O C. Protist O d. Consumer O e. Nutrient QUESTION 16 A salt will dissolve in water to form O a. bases O b. acids O c. buffers O d. ions other than H* and OH" O e. only hydrogen and oxygen bondsarrow_forwardQuestion #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…arrow_forward
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