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QUESTION 6 If you performed the experiment outlined in question 6 with full-leng would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen addec expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1

QUESTION 6 If you performed the experiment outlined in question 6 with full-leng would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen addec expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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question 6,7 are related to question 5 diagram. 

Please answer 6&7 both please

dutea Uu HUie Tormation QUESTION 6 If you performed the experiment outlined in question 6 with full-length N-WASP + Actin + Arp2/3 what actin polymerization kinetics would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen added CDC42 to the reaction, what polymerization curve do you expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1
Transcribed Image Text:dutea Uu HUie Tormation QUESTION 6 If you performed the experiment outlined in question 6 with full-length N-WASP + Actin + Arp2/3 what actin polymerization kinetics would you observe (choose one of the curves in the graph above)? Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1 QUESTION 7 "If you perform the experiment outlined in question 6 and tlen added CDC42 to the reaction, what polymerization curve do you expect to see?" Actin alone Actin+Arp2/3+WHAMM Actin+Arp2/3+WHAMM+2.0xRab1
QUESTION 5 WHAMM is a nucleation promoting factor. Depicted above is a pyrene actin polymerization assay performed with the following reaction conditions 1: Actin + Arp2/3 + WHAMM (active isoform); 2-5: Actin + Arp2/3 + WHAMM (active isoform) and increasing concentrations of Rab1; or 6: actin alone. What effect does Rab1 have on branched actin polymerization? Actin + Arp2/3 + 1.0 500nM WHAMM + 0.2x Rab1(M) + 1.0x Rab1(M) + 2.0x Rab1(M) + 3.0x Rab1(M) Actin alone 0.5 0.0 1000 2000 Time (s) Enhances WHAMM activity inhibits WHAMM activity No effect induces actin bundle formation F-actin (RFU)
Transcribed Image Text:QUESTION 5 WHAMM is a nucleation promoting factor. Depicted above is a pyrene actin polymerization assay performed with the following reaction conditions 1: Actin + Arp2/3 + WHAMM (active isoform); 2-5: Actin + Arp2/3 + WHAMM (active isoform) and increasing concentrations of Rab1; or 6: actin alone. What effect does Rab1 have on branched actin polymerization? Actin + Arp2/3 + 1.0 500nM WHAMM + 0.2x Rab1(M) + 1.0x Rab1(M) + 2.0x Rab1(M) + 3.0x Rab1(M) Actin alone 0.5 0.0 1000 2000 Time (s) Enhances WHAMM activity inhibits WHAMM activity No effect induces actin bundle formation F-actin (RFU)
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