R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to amplified this region - using the primers indicated above and DNA from an individual that was heterozygous - and loaded that PCR sample into the gel below?

You buy a new PCR kit.  The starting concentration for each reagent is as follows: the primers are 200 mM (mmole/L), the PCR buffer + dNTPs is 150 mM (mmole/L), and the polymerase 15 units/mL.      In the protocol manual, it states that you want the final concentration (FC) of primers to be 40 mM each, the FC of PCR buffer + dNTPs to be 25 mM, and to have 4 units of the DNA polymerase in the final 50 mL PCR reaction.

Entire sequence below needs to beamplified by PCR and subcloned into a plasmid vector. Which of the primersequences listed underneath is the correct reverse primer (6 marks)? Copy correctsequence into your answer. Why primer e) is not the right answer (4 marks)? 5'ATCTCTATTTAATATTTATGTCTATTTAAGCCTCATATTTAAAGACAGGGAAGAGCAGAACGGAGCCCCAGGCCTCTGTGTCCTTCCCTGCATTTCTGAGTTTCATTCTCCTGCCTGTAGCAGTGAGAAAAAGCTCCTGTCCTCCCATCCCCTGGACTGGGAGGTAGATAGGTAAATACCAAGTATTTATTACTATGACTGCTCCCCAGCCCTGGCTCTGCAATGGGCACTGGGATGAGCCGCTGTGAGCCCCTGGTCCTGAGGGTCCCCACCTGGGACCCTTGAGAGTATCAGGTCTCCCACGTGGGAGACAAGAAATCCCTGTTTAATATTTAAACAGCAGTGTTCCCCATCTGGGTCCTTGCACCCCTCACTCTGGCCTCAGCCGACTGCACAGCGGCCCCTGCATCCCCTTGGCTGTGAGGCCCCTGGACAAGCAGAGGTGGCCAGAGCTGGGAGGCATGGCCCTGGGGTCCCACGAATTTGCTGGGGAATCTCGTTTTTCTTCTTAAGACTTTTGGGACATGGTTTGACTCCCGAACATCACCGACGCGTCTCCTGCTG 3'a) 5' TTCCGGAAGAAGCTTATACGG 3'b) 5' CTGTGTTCACCTAATATTCCT 3'c) 5' CAGCAGGAGACGCGTCGGTGA 3'd) 5' AGGAATATTAGTATAATCCAC 3'e) 5' GACGCGTCGGTGATGTTCGGG 3’f) 5'…

Calculate the volume (in µL) of DNA template required to add 60.0 ng to a 40.0 µL PCR if the DNA template is at a concentration of 28.0 ng µL-1.

The gel below shows results for the bitter tasting SNP analysis from a CH306 class from a few years ago. Analyze the results and annotate the gel to indicate the bitter tasting ability and homozygous/heterozygous status of each of the individuals represented on the gel. There are three lanes of markers, and the first non-maker lane on the left of the gel is an uncut control PCR product (i.e. there are a total of 10 individuals on the gel starting in lane 3).