Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. (A) To investigate whether the microRNA-145 (miR-145) plays a role in gastric cancer, levels of Ets1 and miR-145 in cancer and normal adjacent tissue of 84 patients were determined by qPCR. (B) Levels in normal (GES-1) and cancer cell lines were determined. (C) Cancer cell lines were transfected with the Ets1 3'-UTR reporter plasmid and either miR-145 or negative control (mock), and luciferase assays were carried out. (D) Western blots of cells transfected with miR-145 or mock stained for Etsl
Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. (A) To investigate whether the microRNA-145 (miR-145) plays a role in gastric cancer, levels of Ets1 and miR-145 in cancer and normal adjacent tissue of 84 patients were determined by qPCR. (B) Levels in normal (GES-1) and cancer cell lines were determined. (C) Cancer cell lines were transfected with the Ets1 3'-UTR reporter plasmid and either miR-145 or negative control (mock), and luciferase assays were carried out. (D) Western blots of cells transfected with miR-145 or mock stained for Etsl
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please help me with the Cellular Biology question below. It is one question, the data given is to help answer it.
Please be as detailed as possible.
Transcribed Image Text:Refer to the experimental approach (i.e., very briefly describe) and give the results AND
conclusions. The answer is incomplete without reference to all the data including controls.
(A) To investigate whether the microRNA-145 (miR-145) plays a role in gastric cancer, levels of
Ets1 and miR-145 in cancer and normal adjacent tissue of 84 patients were determined by qPCR.
(B) Levels in normal (GES-1) and cancer cell lines were determined.
(C) Cancer cell lines were transfected with the Ets1 3'-UTR reporter plasmid and either miR-145
or negative control (mock), and luciferase assays were carried out.
(D) Western blots of cells transfected with miR-145 or mock stained for Ets1
A
B
Relative Ets1 Iranscript
levels (by qPCR)
C-
10.0
8.0
6.0
4.0 -
2.0
0.0
P<0.0001
Normal Cancer
(n = 84) (n = 84)
SV40
Relative luciferase activity
(Renilla /firefly ratio)
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Wt
hRluc Ets1 3¹-UTR
Relative mir-145 expression
(normalized to U6)
WT (nt 2872-2894) 5'-ggugggugguuuauacACUGGAa-3'
Mut (nt 2872-2894) 5'-ggugggugguuuauacACUUU Aa-3'
SGC-7901
Mut
7.0
6.0
5.0
4.0
3.0
Wt
2.0
1.0
0.0
hLuc
Normal Cancer
(n=84) (n = 84)
MKN-45
Mock
miR-145
P<0.0001
Mut
D
Relative transcript levels
(by qPCR)
51
Mock
3
SGC-7901
GES-1
miR-145
miR-145
Ets1
AGS
SGC-7901
MKN-45
Mock
MKN-45
miR-145
Ets1
B-Actin
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