RNA polymerases generally require a primer to begin transcription. (T) (F) The Death Cap Mushroom Amanita phalloides is toxic because of its ability to produce alpha-amanitin, which is an inhibitor of RNA Polymerases I and III. (T) (F) In bacteria, transcription and translation can occur simultaneously. (T) (F) In eukaryotes, transcription and translation can occur simultaneously (T) (F) RNA polymerase II has no form of proofreading activity. (T) (F) Sigma factors specify binding of bacterial RNA Polymerases to specific promoters (T) (F) An E. coli strain with mutations in genes encoding both the dam methylase and the RecA protein would likely be inviable (dead) (T) (F) An E. coli culture grown in a pure (100%) N2 atmosphere would likely have a lower rate of mutations than a culture grown under normal conditions (~30% O2 and 70% N2) (T) (F) Non-homologous end joining repairs double strand DNA breaks with no loss of information, restoring the original sequence. (T) (F) A culture of yeast runs out of Nitrogen and stops growing. Under these conditions, you would expect RNA Pol I to be highly active. (T) (F) Unlike DNA, RNA is synthesized in the 3’ → 5’ (T) (F) An E. coli strain deficient in the dam methylase would probably not be able to properly regulate the initiation of DNA replication. (T) (F) Adding a chemical inhibitor of DNA topoisomerases to an E. coli culture would likely have an effect on the rate of DNA synthesis, but not on the rate of RNA synthesis (T) (F) The DNA sequence upstream of a transcriptional start site (promoter) usually has consensus sequences centered at the -10 and -35 positions, but it turns out that altering this spacing has little effect on sigma factor binding. (T) (F) The C-terminal domain of eukaryotic RNA Pol II is phosphorylated when the polymerase is actively transcribing a gene (T) (F)

Question
Asked Apr 7, 2019
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  1. RNA polymerases generally require a primer to begin transcription. (T) (F)

 

  1. The Death Cap Mushroom Amanita phalloides is toxic because of its ability to produce alpha-amanitin, which is an inhibitor of RNA Polymerases I and III. (T) (F)

 

  1. In bacteria, transcription and translation can occur simultaneously. (T) (F)

 

  1. In eukaryotes, transcription and translation can occur simultaneously (T) (F)

 

  1. RNA polymerase II has no form of proofreading activity. (T) (F)

 

  1. Sigma factors specify binding of bacterial RNA Polymerases to specific promoters (T) (F)

 

  1. An E. coli strain with mutations in genes encoding both the dam methylase and the RecA protein would likely be inviable (dead) (T) (F)

 

  1. An E. coli culture grown in a pure (100%) N2 atmosphere would likely have a lower rate of mutations than a culture grown under normal conditions (~30% O2 and 70% N2) (T) (F)

 

  1. Non-homologous end joining repairs double strand DNA breaks with no loss of information, restoring the original sequence. (T) (F)

 

  1. A culture of yeast runs out of Nitrogen and stops growing. Under these conditions, you would expect RNA Pol I to be highly active. (T) (F)

 

  1. Unlike DNA, RNA is synthesized in the 3’ → 5’ (T) (F)

 

  1. An E. coli strain deficient in the dam methylase would probably not be able to properly regulate the initiation of DNA replication. (T) (F)

 

  1. Adding a chemical inhibitor of DNA topoisomerases to an E. coli culture would likely have an effect on the rate of DNA synthesis, but not on the rate of RNA synthesis (T) (F)

 

  1. The DNA sequence upstream of a transcriptional start site (promoter) usually has consensus sequences centered at the -10 and -35 positions, but it turns out that altering this spacing has little effect on sigma factor binding. (T) (F)

 

  1. The C-terminal domain of eukaryotic RNA Pol II is phosphorylated when the polymerase is actively transcribing a gene (T) (F)

 

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Expert Answer

Step 1

As per the multiple questions policy, I am attempting the very first question posted by you. Kindly, post the other questions separately. The given question asks to determine whether the following statement is true or false. RNA polymerases generally require a primer to begin transcription.

Step 2

RNA polymerase is an important enzyme of the transcription process. Transcription is the initial step of gene expression. In this step, a specific DNA segment is copied into RNA. It is known as messenger RNA or mRNA. RNA polymerase catalyzes the RNA chains initiation and elongation from a DNA template.

Step 3

The active site of RNA polymerase is proficient of initiating the polymerization at a promoter sequence. It forms a transcription initiation complex. This complex is formed by the association of accessory proteins (transcription factors) along with RNA polymerase. This complex is n...

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