S2 A 3. The figure below represents a two-generation pedigree representing the inheritance of hereditary deafness (HD), which, has been shown to be autosomal recessive. Below the pedigree (and aligned with each person in each generation) are Southern blot gels for two RFLP loci. The BamH1 RFLP locus has alleles of either 7 kb or 5 kb and the EcoRI RFLP locus gives either a 4 kb or 3 kb EcoRI fragment. Determine if HD is linked to either of these markers. a. If linkage is detected between HD and one of the RFLPS, are there any recombinant offspring that you b. can detect? If linkage is detected between HD and one of the RFLPs, what is the approximate map distance between the two? Do you think that this distance will efficiently and effectively allow the RFLP to track the HD C. gene? I II 1 2 3 4 5 6 7 8 9 10 11 12 7 kb BamHI SS8 5 kb 4 kb 3 kb EcoRI

Question

The figure below represents a two-generation pedigree representing the inheritance of hereditary deafness (HD), which, has been shown to be autosomal recessive. Below the pedigree (and aligned with each person in each generation) are Southern blot gels for two RFLP loci. The BamH1 RFLP locus has alleles of either 7 kb or 5 kb and the EcoRI RFLP locus gives either a 4 kb or 3 kb EcoRI fragment.

a. Determine if HD is linked to either of these markers.

b. If linkage is detected between HD and one of the RFLPs, are there any recombinant offspring that you can detect?

c. If linkage is detected between HD and one of the RFLPs, what is the approximate map distance between the two? Do you think that this distance will efficiently and effectively allow the RFLP to track the HD gene? 

S2
A
3. The figure below represents a two-generation pedigree representing the inheritance of hereditary deafness
(HD), which, has been shown to be autosomal recessive. Below the pedigree (and aligned with each person in
each generation) are Southern blot gels for two RFLP loci. The BamH1 RFLP locus has alleles of either 7 kb or 5
kb and the EcoRI RFLP locus gives either a 4 kb or 3 kb EcoRI fragment.
Determine if HD is linked to either of these markers.
a.
If linkage is detected between HD and one of the RFLPS, are there any recombinant offspring that you
b.
can detect?
If linkage is detected between HD and one of the RFLPs, what is the approximate map distance between
the two? Do you think that this distance will efficiently and effectively allow the RFLP to track the HD
C.
gene?
I
II
1 2 3 4 5 6 7 8 9 10 11 12
7 kb
BamHI
SS8
5 kb
4 kb
3 kb
EcoRI
Expand
Transcribed Image Text

S2 A 3. The figure below represents a two-generation pedigree representing the inheritance of hereditary deafness (HD), which, has been shown to be autosomal recessive. Below the pedigree (and aligned with each person in each generation) are Southern blot gels for two RFLP loci. The BamH1 RFLP locus has alleles of either 7 kb or 5 kb and the EcoRI RFLP locus gives either a 4 kb or 3 kb EcoRI fragment. Determine if HD is linked to either of these markers. a. If linkage is detected between HD and one of the RFLPS, are there any recombinant offspring that you b. can detect? If linkage is detected between HD and one of the RFLPs, what is the approximate map distance between the two? Do you think that this distance will efficiently and effectively allow the RFLP to track the HD C. gene? I II 1 2 3 4 5 6 7 8 9 10 11 12 7 kb BamHI SS8 5 kb 4 kb 3 kb EcoRI

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