Show by serial dilution how you would prepare a 1:800 dilution of a protein solution. Assume the largest piece of measuring equipment is a 10-ml glass pipette.
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- Identify and explain the mistakes made in the following laboratory protocols: A 10.00 mM solution of glucose (FW 180.16 g/mole) was prepared by first adding 180.16 mg of glucose to a 100 mL empty volumetric flask, and then DI H2O was added to the volumetric flask until the meniscus reached the 100 mL volume mark. The flask was capped and the solution vigorously mixed until the glucose completely dissolved.A calibration curve of NaCl conductivity (y-axis) vs concentration (mass percent, x-axis) is made. The equation of line of best fit is y = 459.1x + 0.306 and the R 2 is 0.9998. Based on this data what is the mass percent of an unknown NaCl solution with a conductivity of 1,566 uS/cm? 4.54 % 4.46 % 5.03 % 3.97 %you have 10mL of a 60% stock dye solution. what is the maximum amount of a 12% dye solution you could prepare? how would you go about preparing the 12% dye solution?
- A student creates a calibration plot for serial dilutions of Cu(NO3)2. A graph of concentration in M (x-axis) versus absorbance (y-axis) gives a linear trendline of y = 0.345x - 0.11. c.) How many grams of Cu(NO3)2 are present in 100 grams of solution? How many grams of water?What is the concentration, in mg/mL, of a solution containing 2 mEq of potassium chloride per mL? MW = 75 200mg/mL 150mg/mL 1500mg/mL 1800mg/mLProcaine hydrochloride (MW = 273; L iso value = 3.37). Calculate its NaCl Equivalent
- Compounds used in pharmacology experiments are often initially made as stock solutions. Stock solutions can then be diluted to the desired final concentration. For instance, take Serotonin (5-HT) which was initially made at a 15 mM stock concentration. If an experiment requires 85 mL of Serotonin at a concentration of 5 μM. What volume of stock solution needs to be diluted? Show your workings.What micropipette would you use to measure this volume?Believe it or not, there is gold dissolved in the Earth’s ocean! The concentration can run as high as 40.0 ppb. If you could somehow extract all the gold from 7.45 x 10^6 kg of ocean water, how many grams of gold could you recover? Assume the concentration of gold is 40.0 ppbConcentrated Potassium Chloride is available in a concentration of 1.49 g of KCL per 50 mL. If a 50 mL vial is added to a 1 liter bag of half-normal saline (1/2NS), what is the total concentration, in mEq, of chloride in the final product?
- the labortory procedure specified that the concentration of the HCL solution be 2.0 M, but only 12.3M, but only 12.3M HCL solution was available. Describe the steps for safety preparing 50.0mL OF 2.0M HCL (aq)using 12.3M HCL solution and materials selected from the list below. Show any necessary calculations. 10.0mL graduated cylinder, 25mL pipet, distilled water, 250mL beakers, Balamce, 50.00mL volumetric flask, DropperWhy do Sedimentation and filtration is important in water treatment?Is a period of timeimportant in water purification?You are creating a standard curve for a protein experiment. You have to stock solution of BS a and would like to obtain dilutions. Explain how you would create a 1:1000 dilution of your protein stock solution.