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solated bacteria from the sputum of a patient that seemed somewhat resistant to their antibiotic ce multiple weeks of treatment had yielded no satisfactory improvement of their active disease. er had then resuspended the patient's sputum in saline and used a mouse model to measure the a phage selected for its capacity to target M. mycobacterium, as a single therapy, and as a herapy with antibiotics. Here is a schematic representation of the experiment: Group 1 Control (saline) oculate mice th patient utum Group 2 wait for active disease to show Treated with phage Collect lung sample every 2 weeks Group 3 Treated with Abx Split mice showing active disease in 4 Group 4 Treated with Abx and Count bacteria in sample groups phage itum from patients were isolated and resuspended in saline. Mice were given equal amounts of sputum intra-nasally and were left to develop active disease. Once the mice developed active were split into 4 group: a control group treated with saline (group 1), a group treated with the e only (group 2), a group treated with antibiotics (Abx) only (group 3) and a group treated with both eriophage (group 4). Group 1 was injected with saline (i.v.) every week, to mimic the treatment oup 2 was given a single dose of bacteriophage on day one of active disease, Group3 was treated every week, and Group 4 was treated with one dose of bacteriophage on day 1 of active disease oses of Abx. Once the 4 treatments started, several mice from each group were culled every 2 weeks , and their lung lavage were collected to count the number of bacteria growing on agar plates with esults from this experiment that Ahmed presented at the lab meeting: Control Figure 5. Bacterial growth in mice model of tuberculosis. Mice were inoculated with the same number of M.tuberculosis and treatment with control (saline), Abx, phage, or Abx with phage started at onset of active disease. Mice lung lavage (sputum samples) were collected Abx and phage

solated bacteria from the sputum of a patient that seemed somewhat resistant to their antibiotic ce multiple weeks of treatment had yielded no satisfactory improvement of their active disease. er had then resuspended the patient's sputum in saline and used a mouse model to measure the a phage selected for its capacity to target M. mycobacterium, as a single therapy, and as a herapy with antibiotics. Here is a schematic representation of the experiment: Group 1 Control (saline) oculate mice th patient utum Group 2 wait for active disease to show Treated with phage Collect lung sample every 2 weeks Group 3 Treated with Abx Split mice showing active disease in 4 Group 4 Treated with Abx and Count bacteria in sample groups phage itum from patients were isolated and resuspended in saline. Mice were given equal amounts of sputum intra-nasally and were left to develop active disease. Once the mice developed active were split into 4 group: a control group treated with saline (group 1), a group treated with the e only (group 2), a group treated with antibiotics (Abx) only (group 3) and a group treated with both eriophage (group 4). Group 1 was injected with saline (i.v.) every week, to mimic the treatment oup 2 was given a single dose of bacteriophage on day one of active disease, Group3 was treated every week, and Group 4 was treated with one dose of bacteriophage on day 1 of active disease oses of Abx. Once the 4 treatments started, several mice from each group were culled every 2 weeks , and their lung lavage were collected to count the number of bacteria growing on agar plates with esults from this experiment that Ahmed presented at the lab meeting: Control Figure 5. Bacterial growth in mice model of tuberculosis. Mice were inoculated with the same number of M.tuberculosis and treatment with control (saline), Abx, phage, or Abx with phage started at onset of active disease. Mice lung lavage (sputum samples) were collected Abx and phage

Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter17: Bacterial And Viral Genetics
Section: Chapter Questions
Problem 1ITD
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Ahmed had isolated bacteria from the sputum of a patient that seemed somewhat resistant to their antibiotic treatment, since multiple weeks of treatment had yielded no satisfactory improvement of their active disease. The researcher had then resuspended the patient's sputum in saline and used a mouse model to measure the efficiency of a phage selected for its capacity to target M. mycobacterium, as a single therapy, and as a combination therapy with antibiotics. Here is a schematic representation of the experiment: Group 1 Control (saline) Inoculate mice with patient sputum Group 2 wait for active disease to show Treated with phage Sputum samples Collect lung sample every 2 weeks Tuberculosis patient Group 3 Treated with Abx Split mice showing active disease in 4 Group 4 Treated with Abx and Count bacteria in sample groups phage Figure 4. Sputum from patients were isolated and resuspended in saline. Mice were given equal amounts of resuspended sputum intra-nasally and were left to develop active disease. Once the mice developed active disease, they were split into 4 group: a control group treated with saline (group 1), a group treated with the bacteriophage only (group 2), a group treated with antibiotics (Abx) only (group 3) and a group treated with both Abx and bacteriophage (group 4). Group 1 was injected with saline (i.v.) every week, to mimic the treatment with Abx, Group 2 was given a single dose of bacteriophage on day one of active disease, Group3 was treated i.v. with Abx every week, and Group 4 was treated with one dose of bacteriophage on day 1 of active disease and weekly doses of Abx. Once the 4 treatments started, several mice from each group were culled every 2 weeks until week 16, and their lung lavage were collected to count the number of bacteria growing on agar plates with Abx. Here are the results from this experiment that Ahmed presented at the lab meeting: 150 Control Figure 5. Bacterial growth in mice model of tuberculosis. Mice were inoculated with the same number of M.tuberculosis and treatment with control (saline), Abx, phage, or Abx with phage started at onset of active disease. Mice lung lavage (sputum samples) were collected every 2 weeks for the duration of the infection model (16 weeks). Each mouse sputum sample was plated antibiotics to count the number of 100 Abx and phage 50 Phage only agar containing on Abx only growing colonies in the sputum sample. Abx = antibiotics. 2 6 8 10 12 14 16 Time (weeks) Practice Question #10 p. 225 A)Describe the results obtained for each treatment (description without an explanation). B)Do you think the bacteria isolated from the patient is sensitive to the antibiotics? Explain your reasoning. Number of bacteria (colonies per plate)
Transcribed Image Text:Ahmed had isolated bacteria from the sputum of a patient that seemed somewhat resistant to their antibiotic treatment, since multiple weeks of treatment had yielded no satisfactory improvement of their active disease. The researcher had then resuspended the patient's sputum in saline and used a mouse model to measure the efficiency of a phage selected for its capacity to target M. mycobacterium, as a single therapy, and as a combination therapy with antibiotics. Here is a schematic representation of the experiment: Group 1 Control (saline) Inoculate mice with patient sputum Group 2 wait for active disease to show Treated with phage Sputum samples Collect lung sample every 2 weeks Tuberculosis patient Group 3 Treated with Abx Split mice showing active disease in 4 Group 4 Treated with Abx and Count bacteria in sample groups phage Figure 4. Sputum from patients were isolated and resuspended in saline. Mice were given equal amounts of resuspended sputum intra-nasally and were left to develop active disease. Once the mice developed active disease, they were split into 4 group: a control group treated with saline (group 1), a group treated with the bacteriophage only (group 2), a group treated with antibiotics (Abx) only (group 3) and a group treated with both Abx and bacteriophage (group 4). Group 1 was injected with saline (i.v.) every week, to mimic the treatment with Abx, Group 2 was given a single dose of bacteriophage on day one of active disease, Group3 was treated i.v. with Abx every week, and Group 4 was treated with one dose of bacteriophage on day 1 of active disease and weekly doses of Abx. Once the 4 treatments started, several mice from each group were culled every 2 weeks until week 16, and their lung lavage were collected to count the number of bacteria growing on agar plates with Abx. Here are the results from this experiment that Ahmed presented at the lab meeting: 150 Control Figure 5. Bacterial growth in mice model of tuberculosis. Mice were inoculated with the same number of M.tuberculosis and treatment with control (saline), Abx, phage, or Abx with phage started at onset of active disease. Mice lung lavage (sputum samples) were collected every 2 weeks for the duration of the infection model (16 weeks). Each mouse sputum sample was plated antibiotics to count the number of 100 Abx and phage 50 Phage only agar containing on Abx only growing colonies in the sputum sample. Abx = antibiotics. 2 6 8 10 12 14 16 Time (weeks) Practice Question #10 p. 225 A)Describe the results obtained for each treatment (description without an explanation). B)Do you think the bacteria isolated from the patient is sensitive to the antibiotics? Explain your reasoning. Number of bacteria (colonies per plate)
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