Spro pro loct Short time 00-00. Hfr cell F cll pro pro pro Spro* Transfer of Hfr lac Hfr cell F- reciplent cell lac joc chromosome lac lact pro Longer time -Origin of transfer (toward lac) pro Tpro loct Jact Transfer of bacterial genes from an Hfr cell to an F- cell. The transfer of the bacterial chromosome begins at the origin of transfer and then proceeds around the circular chromosome. After a segment of chromosome has been transferred to the F- recipient cell, it recombines with the recipient cell's chromosome. Genes+Traits The F- recipient cell was originally lac- (unable to metabolize lactose) and pro- (unable to synthesize proline). If conjugation occurs for a short period of time, the recipient cell acquires lac+, allowing it to metabolize lactose. If conjugation occurs for a longer period of time, the recipient cell also acquires pro+, enabling it to synthesize proline.

Spro pro loct Short time 00-00. Hfr cell F cll pro pro pro Spro* Transfer of Hfr lac Hfr cell F- reciplent cell lac joc chromosome lac lact pro Longer time -Origin of transfer (toward lac) pro Tpro loct Jact Transfer of bacterial genes from an Hfr cell to an F- cell. The transfer of the bacterial chromosome begins at the origin of transfer and then proceeds around the circular chromosome. After a segment of chromosome has been transferred to the F- recipient cell, it recombines with the recipient cell's chromosome. Genes+Traits The F- recipient cell was originally lac- (unable to metabolize lactose) and pro- (unable to synthesize proline). If conjugation occurs for a short period of time, the recipient cell acquires lac+, allowing it to metabolize lactose. If conjugation occurs for a longer period of time, the recipient cell also acquires pro+, enabling it to synthesize proline.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter17: Metabolism: An Overview

Section: Chapter Questions

Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...

See similar textbooks

Similar questions

#2 EcoRI --- 5’ G ↓AATTC 3’ 5’ ACG ACGTATTAGAATTCTTA TCCGCCGCCGGAATTCT CATCA 3’ 3’ TGC TGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:
#4 BamI --- 5’ CCTAG ↓G 3’ 5’ ACGCCTAGGACGTATTATCCTAGGTAT CCGCCGCCGT CATCA 3’ 3’ TGCGGATCCTGCATAATAGGATCCATAGGCGGCGGCAGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:
#1 HindII --- 5’ GTC ↓ GAC 3’ 5’ ACGACGTAGTCGACTTATTAT GTCGACCCGCCGCGTGTCGACCATCA 3’ 3’ TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut:
Utilizing Hind III and EcoR V Restriction Enzyme with Pet41 and the following gene of interest... a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781…
The terms conjugation, transduction, and transformation are usedto describe three different natural forms of genetic transferbetween bacterial cells. Briefly discuss the similarities and differencesamong these processes?
Transcribe an mRNA sequence from this TEMPLATE STRAND of DNA 3' CGTACGTGTATCCCATCC 5' 5' GCAUGCACAUAGGGUAGG 3' 5' GCAUGCACAUAGGGUAGG 3' 3' CGUACGUGUAUCCCAUCC 5' 5' GCATGCACATAGGGTAGG 3' 3' CGTACGUGTATCCCATCC 5'
Let’s suppose a new strain of P1 phage has been identified thatpackages larger pieces of the E. coli chromosome. This P1 strainpackages pieces of the E. coli chromosome that are 5 minuteslong. If two genes are 0.7 minute apart along the E. coli chromosome,what would be the cotransduction frequency using a normalstrain of P1 and using the new strain of P1 that packages largerpieces? What would be the experimental advantage of using thisnew P1 strain?
By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?
Transcribe the DNA sequence from HbA mRNA: nucleotides: ACGTU
The DNA sequence of one strand of a gene from threeindependently isolated mutants is given here (5′ endsare at left). Using this information, what is the sequence of the wild-type gene in this region?mutant 1 ACCGTAATCGACTGGTAAACTTTGCGCGmutant 2 ACCGTAGTCGACCGGTAAACTTTGCGCGmutant 3 ACCGTAGTCGACTGGTTAACTTTGCGCG
Redraw Figure 10-6 with the goal of adding one EcoRIend and one XhoI end. Below is the Xhol recognitionsequence.Recognition sequence:. . . CTCGAG . . .. . . GAGCTC . . .After cut:. . . C TCGAG . . .. . . GAGCT C . . .
Origins of transfer can be located in many different locations, and their direction of transfer can be clockwise or counterclockwise. Let’s suppose a researcher conjugated six different Hfr strains that werethr+ leu+ tons strr azis lac+ gal+ pro+ met+ to an F− strain that wasthr− leu− tonr strs azir lac− gal− pro− met−, and obtained the followingresults:Draw a circular map of the E. coli chromosome that shows thelocations and orientations of the origins of transfer in these sixHfr strains.