SR proteins are essential to proper spliceosome assembly and are known to take part in the regulation of alternative splicing. Surprisingly, the role of SR proteins in splice-site selection and alternative splicing is affected by the promoter used for the transcription of the pre-mRNA. For example, through genetic engineering, RNA polymerase II promoters that have somewhat different sequences can be created. When pre-mRNAs with exactly the same sequences are transcribed from two different RNA polymerase II promoters that differ slightly in sequence, which promoter is used can affect how the pre-mRNA is spliced. Propose a mechanism by which the DNA sequence of an RNA polymerase II promoter could affect alternative splicing of pre-mRNA.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
SR proteins are essential to proper spliceosome assembly and are known
to take part in the regulation of alternative splicing. Surprisingly, the
role of SR proteins in splice-site selection and alternative splicing is
affected by the promoter used for the transcription of the pre-mRNA.
For example, through genetic engineering, RNA polymerase II
promoters that have somewhat different sequences can be created. When
pre-mRNAs with exactly the same sequences are transcribed from two
different RNA polymerase II promoters that differ slightly in sequence,
which promoter is used can affect how the pre-mRNA is spliced.
Propose a mechanism by which the DNA sequence of an RNA
polymerase II promoter could affect alternative splicing of pre-mRNA.
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