The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down?

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
Problem 11RQ
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1. The end product of protein metabolism is often ammonia, a base. What effect will this have on pH, will it go up or down? _________________________________________

2. Another common end product, pyruvic acid. Into which metabolic pathway will the body use pyruvic acid as a substrate to produce ATP?

3. Bacterium Z had the lab results below. What can you tell me about the protein metabolism of this bacterium?

Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan? ________________________________________________

Can it desulfur proteins? _______________________________________

Can it produce NH3 from the amino acid phenylalanine? _____________________________________

4. Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this bacterium?

Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids and tryptophan? ________________________________________________

Can it desulfur proteins? _______________________________________

Can it produce NH3 from the amino acid phenylalanine?

Urea
Gelatin
Phenylalanine
Peptone
agar
Tubes
Slants
Iron Deeps
Slants
Blackening
Result
solid
Fushia
No color
at butt of
tube
Other
possible
none
none
none
none
results
Other
possible
none
none
none
none
results
Which protein and amino acids can it catabolize,
among gelatin, urea, phenylalanine, sulfur-containing
amino acids, and tryptophan?
Can it desulfur proteins?
Can it produce NH3 from the amino acid
phenylalanine?
4. Bacterium Q had the lab results below. What can
you tell me about the protein metabolism of this
bacterium?
Urea
Рeptone
Gelatin
Phenylalanine
agar
Iron
Tubes
Slants
Slants
Deeps
Result
solid
Fushia
No color
yellow
Other
possible
none
none
none
none
results
Other
possible
none
none
none
none
results
Which protein and amino acids can it catabolize,
among gelatin, urea, phenylalanine, sulfur-containing
amino acids and tryptophan?
Can it desulfur proteins?
Can it produce NH3 from the amino acid
phenylalanine?
Transcribed Image Text:Urea Gelatin Phenylalanine Peptone agar Tubes Slants Iron Deeps Slants Blackening Result solid Fushia No color at butt of tube Other possible none none none none results Other possible none none none none results Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids, and tryptophan? Can it desulfur proteins? Can it produce NH3 from the amino acid phenylalanine? 4. Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this bacterium? Urea Рeptone Gelatin Phenylalanine agar Iron Tubes Slants Slants Deeps Result solid Fushia No color yellow Other possible none none none none results Other possible none none none none results Which protein and amino acids can it catabolize, among gelatin, urea, phenylalanine, sulfur-containing amino acids and tryptophan? Can it desulfur proteins? Can it produce NH3 from the amino acid phenylalanine?
To become familiar with the media before lab, please
read both protein catabolism exercise background
information. In particular pay attention to the figures.
Similar to last lab, the table below summarizes the
different metabolic tests. Using the table, interpret the
results in the following questions. There are results to
interpret on the back of this handout.
Urea
Gelatin
Phenylalanine
Рeptone
agar
Tubes
Slants
Iron Deeps
Slants
Protein
or
Gelatin
Amino
Urea
Phenylalanine
Peptone
protein
Acid in
media
Reagent
Iron (III)
added in
none
None
chloride
none
second
(Fe3+)
period
Initial
no color
orange
No color
None/Yellow
color
Negative
result (-)
solid
yellow
по color
yellow
Meaning
Can not
Sulfur
No
Did not
of –
utilize
amino acids
urease
deaminate
result
gelatin
not utililized
Positive
blackening
result
liquid
fushia
green
at butt of
(+)
tube
H2S
Meaning
Catabolized
Urease
Deaminated
produced
of +
gelatin
present
phenylalanine
from sulfur
result
amino acids
3. Bacterium Z had the lab results below. What can
you tell me about the protein metabolism of this
bacterium?
Transcribed Image Text:To become familiar with the media before lab, please read both protein catabolism exercise background information. In particular pay attention to the figures. Similar to last lab, the table below summarizes the different metabolic tests. Using the table, interpret the results in the following questions. There are results to interpret on the back of this handout. Urea Gelatin Phenylalanine Рeptone agar Tubes Slants Iron Deeps Slants Protein or Gelatin Amino Urea Phenylalanine Peptone protein Acid in media Reagent Iron (III) added in none None chloride none second (Fe3+) period Initial no color orange No color None/Yellow color Negative result (-) solid yellow по color yellow Meaning Can not Sulfur No Did not of – utilize amino acids urease deaminate result gelatin not utililized Positive blackening result liquid fushia green at butt of (+) tube H2S Meaning Catabolized Urease Deaminated produced of + gelatin present phenylalanine from sulfur result amino acids 3. Bacterium Z had the lab results below. What can you tell me about the protein metabolism of this bacterium?
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ISBN:
9781133893943
Author:
ESTRIDGE
Publisher:
Cengage