The figure shows a two-dimensional gel of a mixture ofproteins A, B, and C.The vertical axis represents SDS-PAGE separation, run fromtop to bottom. The horizontal axis represents isoelectricfocusing with a pH gradient that runs from left(high pH) toright (low pH).ВAProteins A, B, and C have isoelectric points of 8.2, 6.7, and5.2, respectively.СPlace proteins A, B, and C in order of increasing elutionvolume on a size exclusion column. Assume they arehighpHlowisoelectric focusingmonomers under the conditions of separation.pHSDS-PAGE Place proteins A, B, and C in order of probable increasingelution volume in cation exchange chromatography duringan increasing salt gradient run at pH 5.

Question
Asked Sep 18, 2019
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Proteins A, B, and C have isoelctric points of 8.2, 6.7,  and 5.2 respectively. 

Place proteins A, B, and C in order of increasing volume on a size exclusion column. 

Then place the proteins in order of probable increasing elution volume in cation exchange chromatography during an increasing salt graditend run at pH 5. 

The figure shows a two-dimensional gel of a mixture of
proteins A, B, and C.
The vertical axis represents SDS-PAGE separation, run from
top to bottom. The horizontal axis represents isoelectric
focusing with a pH gradient that runs from left(high pH) to
right (low pH).
В
A
Proteins A, B, and C have isoelectric points of 8.2, 6.7, and
5.2, respectively.
С
Place proteins A, B, and C in order of increasing elution
volume on a size exclusion column. Assume they are
high
pH
low
isoelectric focusing
monomers under the conditions of separation.
pH
SDS-PAGE
help_outline

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The figure shows a two-dimensional gel of a mixture of proteins A, B, and C. The vertical axis represents SDS-PAGE separation, run from top to bottom. The horizontal axis represents isoelectric focusing with a pH gradient that runs from left(high pH) to right (low pH). В A Proteins A, B, and C have isoelectric points of 8.2, 6.7, and 5.2, respectively. С Place proteins A, B, and C in order of increasing elution volume on a size exclusion column. Assume they are high pH low isoelectric focusing monomers under the conditions of separation. pH SDS-PAGE

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Place proteins A, B, and C in order of probable increasing
elution volume in cation exchange chromatography during
an increasing salt gradient run at pH 5.
help_outline

Image Transcriptionclose

Place proteins A, B, and C in order of probable increasing elution volume in cation exchange chromatography during an increasing salt gradient run at pH 5.

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Expert Answer

Step 1

The heavy molecules travel shorter distance whereas light molecules travel larger distance under the influence of electric field. Protein molecules are separated with the help of SDS PAGE. An isoelectric point is the pH of the molecule which carries zero net charge.

Step 2

The movement  and size of the protein molecule is inversely proportional to each other. Protein C has travelled the farthest distance in SDS PAGE thus the size of this protein is smallest. The arrangement of proteins in increasing order of size is  C < A < B.

Step 3

The elution volume is inversely proportional to the molecular weight of proteins. The heavier the protein, the fast will be elusion. Thus, the size of the protein B is the larger and it has high molecular weight so it will elute out fast. Furthermore, th...

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