The figure shows a two-dimensional gel of a mixture of proteins A, B, and C. The vertical axis represents SDS-PAGE separation, run from top to bottom. The horizontal axis represents isoelectric focusing with a pH gradient that runs from left(high pH) to right (low pH). В A Proteins A, B, and C have isoelectric points of 8.2, 6.7, and 5.2, respectively. С Place proteins A, B, and C in order of increasing elution volume on a size exclusion column. Assume they are high pH low isoelectric focusing monomers under the conditions of separation. pH SDS-PAGE Place proteins A, B, and C in order of probable increasing elution volume in cation exchange chromatography during an increasing salt gradient run at pH 5.
The figure shows a two-dimensional gel of a mixture of proteins A, B, and C. The vertical axis represents SDS-PAGE separation, run from top to bottom. The horizontal axis represents isoelectric focusing with a pH gradient that runs from left(high pH) to right (low pH). В A Proteins A, B, and C have isoelectric points of 8.2, 6.7, and 5.2, respectively. С Place proteins A, B, and C in order of increasing elution volume on a size exclusion column. Assume they are high pH low isoelectric focusing monomers under the conditions of separation. pH SDS-PAGE Place proteins A, B, and C in order of probable increasing elution volume in cation exchange chromatography during an increasing salt gradient run at pH 5.
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.65P: 22-65 (a) What is the difference in the quaternary structure between fetal hemoglobin and adult...
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Proteins A, B, and C have isoelctric points of 8.2, 6.7, and 5.2 respectively.
Place proteins A, B, and C in order of increasing volume on a size exclusion column.
Then place the proteins in order of probable increasing elution volume in cation exchange chromatography during an increasing salt graditend run at pH 5.
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