The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.

The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (μg) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 a. use excel, another program, or graph paper to produce a calibration curve based on the data. b. Use linear regression (best fit line) to obtain the slope of the line c. Determine the unknown protein content for a sample that shows an absorbance reading of 0.150.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter14: Chromatography

Section: Chapter Questions

Problem 9P

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A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
I have set up my spreadsheet using this sample data and reproduced the result with a pKa of 4.64. true or false
Often the analyst will compare known quantities of analyte to unknown quantities of the material to be analyzed. This may be done in one of three ways: -use calibration curves; use standard additions; or use internal standards. The method of standard additions would be used when A) the quantity of sample analyzed or the instrument response varies from run to run. B) the standard solutions and the unknown solution all have similar characteristics are unaffected by the other material in the sample. C) the sample composition is unknown or complex and affects the analytical signal.
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.187. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
Plot the following data and perform linear regression to obtain the value of the slope, the value of the y-intercept, and the correlation coefficient r. x- axis y-axis ln(0) 4.6052 0.0000 3.8712 0.6931 3.4012 1.0986 2.7081 1.3863 2.1972 1.6094 1.7918 1.7918 1.0986 1.9459 0.0000
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I have set up my spreadsheet and was able to reproduce the result with a pKa of 4.64. TRUE OR FALSE
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You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.523 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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