The following four compounds are present in a mixture. You want to separatethem using reverse-phase liquid chromatography.OH`NH213a. Predict the order of elution. In no more than two sentences, justifyyour prediction.b. Our ability to separate compounds like the four above are dependenton the properties of the column. Using the data from a 250 mmcolumn shown below, please calculate the retention factor forcompound A, the number of theoretical plates for compound C andthe plate height for compound C.compoundt, (min) w (min)tm5.040.10A7.100.16В7.150.17C7.230.16c. Do you want a large or small number of theoretical plates? In no morethan two sentences, explain why.

Order of elution First 4, then 1, then 2, lastly 3. Justification. As reverse-phase liquid…

The following four compounds are present in a mixture. You want to separate them using reverse-phase liquid chromatography. OH `NH2 2 3 a. Predict the order of elution. In no more than two sentences, justify your prediction.

The following four compounds are present in a mixture. You want to separate them using reverse-phase liquid chromatography. OH `NH2 2 3 a. Predict the order of elution. In no more than two sentences, justify your prediction.

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter91: Gas Chromatography

Section: Chapter Questions

Problem 1P

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Question

100%

The following four compounds are present in a mixture. You want to separate
them using reverse-phase liquid chromatography.
OH
`NH2
1
3
a. Predict the order of elution. In no more than two sentences, justify
your prediction.
b. Our ability to separate compounds like the four above are dependent
on the properties of the column. Using the data from a 250 mm
column shown below, please calculate the retention factor for
compound A, the number of theoretical plates for compound C and
the plate height for compound C.
compoun
d
t, (min) w (min)
tm
5.04
0.10
A
7.10
0.16
В
7.15
0.17
C
7.23
0.16
c. Do you want a large or small number of theoretical plates? In no more
than two sentences, explain why.

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