The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data isshown below: Cuvette 1 is the clankCuvette #ml albuminAbsorbance0.0.2.06330.4.1634.0.6.1870.8.2531.0.289Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find thecorrelation coefficient (r). R2 > 0.99 = excellent data; r2 0.98-0.95 good data r2 0.94 -0.90 =fair data. How would you describe this data?-To find the relative sensitivity, for each of tubes 2-6 (neglect blank), perform the followingoperation absorbance (Lowry)/Absorbance (Biuret x 0.5 gram albumin (Biuret)/0.05 gram(Lowry) x 200 ml (Lowry)/100 mL (Biuret). This implifies to Relative sensitivity = 20 dilutionfactor x absorbance (Lowry)/Absorbance (Biuret). Report your relative sensitivity as an averageof all 5 values.

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The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 1 2 0.2 .063 3 0.4 163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 excellent data; r2 0.98-0.95 good data r2 0.94-0.90 = fair data. How would you describe this data?-

The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 1 2 0.2 .063 3 0.4 163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 excellent data; r2 0.98-0.95 good data r2 0.94-0.90 = fair data. How would you describe this data?-

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.12QAP

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The nitrate in water was analyzed using a spectrophotometric method. The absorbances of the standards and the unknown sample were obtained at 550 nm.Solution Absorbance 2.00 µM 0.0656.00 µM 0.20510.00 µM 0.33814.00 µM 0.47418.00 µM 0.598Unknown 0.402 Write the equation of the lineWhat is the standard deviation of the calibration curve?
Maria was tasked to determine the concentration of a Fe2(SO4)3 solution. She prepared 5 different standards of Fe2(SO4)3 using the table below as her guide. She ran the standards and the unknown solution through UV-VIS spectroscopy and recorded the absorbances of each solution. HINT: use the dilution equation to determine the concentration of Fe2(SO4)3 in each test tube before preparing the standard curve. NOTE: No need to force the line to pass through zero. Just graph the data as is. What is the slope of the line of the standard curve from the given data? What is the concentration of the unknown solution?
A toluene solution (1.50 mg / L) shows an absorbance of 0.30 in a cuvette with a 0.025 mm optical path. Calculate the limit of detection (in ppb) for toluene if the noise with the solvent spectrum is 0.002 absorbance units in a 0.050 mm cuvette.
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