This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka of PNP based on different pHs. I have a sigmoid graph of the absborances of PNP at different pHs at 400 nm. I have pH on the x-axis and absorbance on the y-axis. How do a calculate the pka based off of this graph?

This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka of PNP based on different pHs. I have a sigmoid graph of the absborances of PNP at different pHs at 400 nm. I have pH on the x-axis and absorbance on the y-axis. How do a calculate the pka based off of this graph?

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter3: Statistical Tests With Excel

Section: Chapter Questions

Problem 9P

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