Question

This is a Biochemistry lab question. For the lab we are spectrophotometrically determining the pka of PNP based on different pHs. I have a sigmoid graph of the absborances of PNP at different pHs at 400 nm. I have pH on the x-axis and absorbance on the y-axis. How do a calculate the pka based off of this graph?

Expert Answer

Want to see the step-by-step answer?

Check out a sample Q&A here.

Want to see this answer and more?

Experts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!*

*Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers.
Tagged in
Science
Chemistry

Related Chemistry Q&A

Find answers to questions asked by students like you.

Q: In normal blood, there are about 5.4×109 red blood cells per milliliter. The volume of a red blood c...

A: Click to see the answer

Q: Which properties would be ideal in selecting the compound to be used as a primary standard for quant...

A: In quantitative volumetric analysis, hygroscopic, expensive, some purity, solid and stable are the i...

Q: organic chemistry, what will be the mechanisms of these two? SN1 or SN2? why?

A: The mechanism followed here might be SN1.SN1 reaction occurs through 2 steps. In first step, removal...

Q: A. From the graph, estimate the normal boiling point of substance X.  B. From the graph above, estim...

A: Boiling occur when vapor pressure become equal to atmospheric pressure. So, boiling point of each ca...

Q: Predict the product obtained when 1-pentyne reacts with NaNH2 in NH3 followed by MeI

A: 1-pentyne is a terminal alkyne and the reagent sodamide can deprotonate the terminal hydrogen. Methy...

Q: 3 Calculate the energy needed to convert 0.75 mol of liquid water at 15°C to steam at 130°C. = 4.18 ...

A: Given,Moles of liquid water = 0.75 molInitial temperature of water = 15.0°CFinal temperature of stea...

Q: You placed 6.35 g of a mixture containing unknown amounts of BaO(s) and MgO(s) in a 3.50-L flask con...

A: Convert the given temperature that is 30 degree Celsius in kelvin as shown follows:T (K) = (30 + 273...

Q: Enter the symbol of the element which would have 2 electrons and -1 charge

A: The symbol of the element which would have 2 electrons and -1 charge is to be determined.

Q: What is the difference between ionic bonding and covalent bonding?

A: Difference between ionic and covalent bonding:Ionic compounds are generated by the strong electrosta...

Q: A student sets up the following equation to convert a measurement. (The ? stands for a number the st...

A: Unit conversions involve conversion of a unit of a physical quantity into another unit of same physi...

Q: A sheet of Aluminum (Al) foil has a total area of 1.000 ft2. The mass of that Al foil is 3.636g. The...

A: Calculate the volume of aluminum foil:

Q: When sodium chloride dissolves in water, how do the water molecules orient around the ions? Select ...

A: The salt will be completely dissociated in the water because salt and water are polar in nature. As ...

Q: A low dose aspirin tablet contains 82 mg of aspirin how many tablets can obtain from 50.g  of aspiri...

A: Given that a tablet contains 82 m g of aspirin. The mass has to be converted into grams.

Q: Convert the following values and write answers in scientific notation, where appropriate. 67 L to qt

A: Given:Conversion of 67 L to qt

Q: How many grams are in 5.32 x 10^22 molecules of CO2

A: Given,Molecules of CO2 = 5.32*1022 molecules. Molar mass of CO2 = 44.01g/mol.

Q: What are the important diagnostic peaks in this IR spectra? Fingerprint region <1500cm^-1 is not ...

A: The important diagnostic peaks in given IR spectra is given in step two.

Q: • 3. Two solutions are prepared using the same solute: Solution A: 0.14 g of the solute dissolves in...

A: When a non-volatile solute is added to a solvent, then occurs a decrease in the freezing point of so...

Q: The mole fraction of silver nitrate, AgNO3, in an aqueous solution is 1.83×10². %. The percent by ma...

A: Given that mole fraction of silver nitrate is 1.83×10-2. The mass percent can be obtained by simply ...

Q: 1) convert 0.66 ibs to grams. 2)convert 0.00534 in cubed to cubic decimeter. 3) convert 2.7 g/cm^3 t...

A: 1.Conversion of 0.66lb to grams.

Q: Could you please help me with this I do not understand it. Explain and show answers clearly!

A: The given questions consists of three multiple questions. We will only solve the first part of the q...

Q: A sample of H2SO4 contains 2.02 g of hydrogen, 32.07 g of sulfur, and 64.00 g of oxygen. How many gr...

A: Given information:First sample of H2SO4 contains 2.02 g of hydrogen, 32.07 g of sulfur, and 64.00 g ...

Q: Acids and Bases 5. Draw the conjugate acid for each of the following. NH3 Он Н-О

A: A pair of substance that is related to each other by donating ad accepting of a single proton is kno...

Q: Draw 6 water molecules in each beaker below.  On the left should be 6 liquid water molecules. On the...

A: The rate of evaporation is inversely proportional to strength of intermolecular forces.  Solids have...

Q: What keeps the particles in a colloidal dispersion from coalescing?

A: Electrostatic repulsion.

Q: 1) Br 2) HS. 3) 4) 5) NaSH Brz ВнутHF РBrз (CH3),CO NABH4 H20, H2SO4 НЕг H2O2, NaOH, H20 CH3CO0

A: 2-bromopropane is first converted into propene. The conversion is done by the use of tertiary alcoho...

Q: please provide in depth explainations as I will be using this to study.

A: Hydrogen bonding can take place between a highly electronegative atom and hydrogen. Intramolecular h...

Q: 11. Compare the two protons shown in the following compound. Which one is more acidic? Explain why.

A: The possible resonance structures after removal of proton is given below.

Q: PLEASE provide an indepth explaniton as I will be using this to study

A: The boiling point will be high for the compound having strong weak intermolecular forces. If the fun...

Q: Barium metal (d = 3.51 g/cm³) has a body-centered cubic structure. Calculate the edge length of a un...

A: It is given that:Density of barium metal = 3.51 g/cm3

Q: PLEASe provide in depth explanitons I will be using this to study

A: The boiling point will be high for the compound having strong weak intermolecular forces. All the gi...

Q: The density of palladium (Pd) is 12.0 g/mL and the cell volume is 5.89 ✕ 10−23 mL. Determine the num...

A: Click to see the answer

Q: how do you convert 3.8 Km/s to m/year     step by step. please and thank you!

A: Click to see the answer

Q: Periodic Table Calculator 7 of 24 Chemistry: Fundamentals and Principles preserted by Saplng Learnin...

A: Click to see the answer

Q: Since different isotopes of the same element have the same chemical behavior, how can they be separa...

A: Click to see the answer

Q: What is osmosis? What is osmotic pressure?

A: Osmosis:From low concentrated solution, where the solvent molecules go through a semipermeable membr...

Q: What's the pH of the solution? See attached image. Please show steps.

A: Click to see the answer

Q: The density of osmium (the densest metal) is 22.57 g/cm3. What volume would be occupied by 2.74 kg o...

A: The volume occupied by 2.74 kg of osmium is calculated as,

Q: When a solution containing 8.00 g of NaOHNaOH in 50.0 g of water at 25.0 ∘C is added to a solution o...

A: The heat released by the reaction can be calculated as follows:

Q: Calculate the solubility of argon in water at an atmospheric pressure of 0.360 atm (a typical value ...

A: Atmospheric pressure is 0.360atm.From the table mole fraction of argon is 9.34×10-3.

Q: A NaOH solution is labeled as 6.75 M, what is its molality? Density of the solution is measured at 1...

A: Given that the molarity of the solution is 6.75 M and the density of the solution is given as 1.04g/...

Q: 1-butanol is reacted with PCC and carried out with an anhydrous solvent DCM, what is the intermediat...

A: PCC can oxidize the alcohol.

Q: The energy of the van der Waals bond, which is responsible for a number of the characteristics of wa...

A: (a) It is given that the van der Waals bond energy for water is 0.50 eV. The expression for translat...

Q: How would a system undergoing adiabatic reversible expansion be represented by the equation for the ...

A: The process where heat and mass do not transfer between thermodynamics system and surrounding is ter...

Q: On the diagram below label regions in which only liquid, only gas and both these phase coexists.

A: In the diagram region A => only liquid

Q: A chemist adds 195.0mL of a 1.5M barium acetate (Ba(C2H3O2)2) solution to a reaction flask. Calculat...

A: Molar mass of barium acetate = 255.43 g/mol

Q: How do you tell whether an element on the periodic table is positive or negative?

A: The elements present in the periodic table originally does not possess any charge and are neutral in...

Q: If the energy of the H2, covalent bond is –4.48 eV, what wavelength of light is needed to break that...

A: Given data,The energy of the H2 covalent bond is -4.48eV.The minimum energy of the photon to break t...

Q: Calculate the amount of energy needed to convert 19.9  g of water at 28.0 °C to steam at 100.0°C.

A: When a substance undergoes transition from liquid to gaseous state, firstly whole of the liquid abso...

Q: A vessel of volume 11.2 L contains 2.0 mol of H2 (g) and 1.0 mol of N2 (g) at 273.15K. Calculate the...

A: Given information:Volume of vessel is 11.2 LMole of H2 is 2.00 molMole of N2 is 1.0 molTemperature i...

Q: Calculate the mass of ethanol in 1.83 qt of ethanol. The density of ethanol is?

A: Calculate the volume of ethanol: