Asked Sep 18, 2019

To make up a solution of phosphate buffered saline (PBS), you need 10 mM Na2HPO4 (anhydrous) (FW: 141.96 g/mol), 3 M NaCl (FW: 58.44 g/mol), and 5mM KH2PO4 (FW: 136.09 g/mol). How many grams of each will you need to make up 850 mL of PBS?


Expert Answer

Step 1

Convert the constituents in grams by multiplying with the respective molarity to convert grams per mole into grams present in 1000 millilitres of solution.

10 mM Na2HPO4 = 141.96 g/mol in grams is 1.4196 grams; 3 M NaCl = 58.44 g/mol in grams is 175.32 grams and 5 mM KH2PO4 = 136.09 g/mol in grams is 0.680 grams.

Step 2

Now, we have the grams of the substance present in 1000 ml of the solution, we need to find the grams of the const...

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