
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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BIOC 385
Biochemistry of RNA Synthesis
Q11.1: Transcriptional initiation at defined sequences in DNA is required to ensure that the 5' end of the RNA transcript is correct. Describe the two types of experimental approaches that have been used to identify regulatory DNA sequences located near gene start sites.
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- Q6. How many exons does tra-RA contain? Q7. How many introns does tra-RA contain?arrow_forwardPreparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?arrow_forward
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