Transformation of bacteria can be done via Electroporation Calcium chloride treatment, heat shock and then on ice Both options are possibilities
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- A broth culture is initially inoculated with a single bacterial cell. For the first hour the bacteria has a generation time of 30 minutes, for the second hour the generation time has been reduced to 15 minutes, and for the third hour the generation time has been reduced to 10 minutes. At the end of the third hour how many bacteria are present in the broth culture?Blood agar media is a _________. Selective media Differential media Rich media Enriched media a) I only b) I and II c) II, III, and IV d) III and IV e) II onlyMacConkey agar plates represent ________ medium. a differential a minimal a selective both a differential and a selective both a minimal and a selective
- 2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Need help list three N-removal Tertiary treatment approaches in order of decreasing cost of operation. State the microbiology (taxa and process) that is respinsible for the lowest cost process.
- Give the use and advantages of the following cultures for the examination ofcolony characteristics: a. Agar plateb. Agar slantc. Broth culturesFrom figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginos
- MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.PLEASE answer the 2 with no much explanation Culture media is sterilized after the inoculation of microbes to ensure optimal growth of target microorganisms True False Microwave oven available in the laboratory can be used to heat culture media, as well as food for lunch. True FalseSweet sorghum juice is used as sole carbon source for ethanol production by Saccharomyces cerevisiae. The overall in situ sterilisation duration is 40 minutes at 120 0C for a pilot reactor (50 L) which is filled with 60% of working volume. The non-sterile medium consists of 3.28 x 106 contaminants L-1. During the autoclaving process, you have noticed that the time taken for heating and cooling are 18 minutes and 15 minutes, respectively. As a process engineer, explain if you agree with this sterilisation practice by using quantitative analysis.