Under certain conditions, peptide bond formation rather than peptide bond hydrolysis is thermodynamically favorable. Would you expect chymotrypsin to catalyze peptide bond formation? Explain.

Q: Some of the following four amino acids : alanine, arginine, histidine, aspartic acid would provide a…

A: Acid-Base Catalysis is involved in the reaction mechanisms that requires the transfer of a proton…

Q: Why is chitin water insoluble but the N-acetyl- D-glucosamine from which it is made water soluble?

A: Biomolecules are organic molecules that function inside living systems. They play a whole repertoire…

Q: Based on the structure of CBB G-250, explain why it can bind to proteins having basic and…

A: CBB G-250 (Coomassie Brilliant Blue G-250) is a dye used to visualize proteins. There are two types…

Q: If isolated regulatory subunits and catalytic subunits of ATCase are mixed, the native enzyme is…

A: ATcase (Aspartate transcarbamoylase) is an allosteric enzyme. It is involved in regulation of…

Q: What kind of inhibitor is threo-sphingosine? Explain this type of inhibition.

A: Introduction:  Enzymes are the catalyst of life. Enzyme inhibitor is defined as a substance which…

Q: Which types of ion exchange resin will the the peptide Ala-Glu-lle-Lys- Leu-Asp-Gly bind to at the…

A: Ion exchange chromatography consists of column with loaded resin that can exchange oppositely…

Q: Histidine is frequently used as a general acid or base in enzyme catalysis. Considering the pKa…

A: Histidine is an essential amino acids with unique acid / base properties. -It is a precursor of…

Q: Which one of the following would best allow us to conclude that the rate of chymotrypsin was…

A: Chymotrypsin is a serine protease enzyme that aids in digestion in several organisms including…

Q: Give full reaction for the synthesis of Cetirizine ?

A: Histamine receptors are transmembrane receptors that generate cellular responses to histamine, a…

Q: An inhibitor that specifically labels chymotrypsin at histidine 57 is Ntosylamido-l-phenylethyl…

A: Proteases are enzymes that can degrade other proteins. Serine proteases are a type of proteases that…

Q: Fibrinogen contains tyrosine- O - sulfate. Propose an activated form of sulfate that could react in…

A: Tyrosine O-sulfation of protein is a post-translational modification that mostly occurs in…

Q: An inhibitor that specifically labels chymotrypsin at histidine 57 is N-tosylamido-l-phenylethyl…

A: Trypsin is an enzyme that helps to digest protein. It breaks down the process of digestion that…

Q: A decapeptide was isolated from a thermophilic bacteria. Treatment of this peptide with 2-…

A: Proteins are large biomolecules that compose structural and motor elements of a cell, and also as…

Q: Chymptrysin preferentially binds thetransition state. Explain what is meant by this.

A: Chymotrypsin is defined as a “hydrolytic enzyme”. It is a member of the super-family, belonging to…

Q: Similarly, hypoxanthine (HX) can be used to label purine residues. As in Problem 4, write reactions…

A: It is given that hypoxanthine can be used to label the purine residues. Hypoxanthine always pair…

Q: What Role Does Transition-State Stabilization Play inEnzyme Catalysis?

A: The enzymes are the protein molecules used to catalyze a chemical reaction by speeding up the rate…

Q: The following data describe the catalysis of cleavage of peptide bonds insmall peptides by the…

A: Elastase is a serine protease produced by the pancreas that catalyzes the cleavage of carboxyl…

Q: An enzyme that catalyzes disulfide– sulfhydryl exchange reactions, called protein disulfide…

A: Protein disulfide isomerase or PDI is an enzyme that breaks sulfide bonds and is found in the…

Q: When specific conditions are met, the creation of peptide bonds rather than the hydrolysis of…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: dentify and describe how you would PEGylate this peptide at its N- terminal amine. Discuss the…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: Draw the structure of the PTH derivative product you would obtain by Edman degradation of the…

A: Phenyl isothiocynate is the Edman reagent. It reacts with N-terminal amino acid to give phenyl…

Q: Provide a simple sketch of the covalent intermediate likely to form between active site serine…

A: DPCC: Diphenylcarbamyl chloride has two phenyl group attached to carbamyl chloride. Serine Protease:…

Q: Arginine exists in two high-pH forms instead of the usual one. Explain why.

A: Amino acids are compounds containing carbon, hydrogen, oxygen and nitrogen. They are monomers…

Q: pyrenylmaleimide could react with which amino acids? Why the lifetime of a prtein will reduce once…

A: Pyrene Malemide is a chemical which is applied as fluorophore to make protein conjugates by…

Q: The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation energy…

Q: Note:- Provide detailed explanation for the reaction that involves the removal of nitrogen from an…

A: Alpha (α)-amino acid: A core C-atom, denoted Cα, is bound to two carboxylic groups, COOH and the…

Q: Which of the following amino acid residues would not provide a side chain for acid- base catalysis…

A: Introduction: Nearly one-third of all known enzymes require metal ions for the catalytic activity…

Q: Would you expect lysozyme to hydrolyze cellulose? Why or why not?

A: Lysozyme is an antimicrobial enzyme that forms a part of the innate immunity. It is also called…

Q: Individuals with phenylketonuria (PKU) are sensitive to phenylalanine in their diet. Why is a…

A: Phenylketonuria (PKU) is considered a rare genetic disorder, which is not able to utilize the amount…

Q: What is the first thing that must occur in order for the catalysis of the peptide bond to begin.…

A: Macromolecules are large organic molecules composed of thousands of covalently connected atoms and…

Q: Discuss the reaction conditions you would need to carry out the PEGylation reaction of this peptide…

A: In order to solve a variety of biopharmaceutical inadequacies, the word "PEGylation" refers to the…

Q: N -Acetylglutamate is required as a cofactor in the synthesis of carbamoyl phosphate. How is…

A: N-acetyl (NAG) glutamate is considered as a cofactor, in the synthesis of the carbamoyl phosphate,…

Q: Why do structural analogs of the transition-state intermediate of an enzyme inhibit the enzyme…

A: Transition state structural analogs of enzymes are chemicals with a structure that resembles the ES…

Q: Azaserine inhibits amidotransferases. State the purine precursor that resembles azaserine. Which…

A: Cancer is the abnormal growth and multiplication of normal cells. Cancer cells divide rapidly.…

Q: . Radioactive uracil can be used to label all of the pyrimidine residues in DNA. Using either names…

A: When the radioactive adenine, particularly uracil, was used as the precursors, highly labeled DNA…

Q: In a paragraph form, provide the experimental procedures in removal of the carbon dioxide present in…

A: Proteins are composed of amino acids in which amine group of one amino acid linked to carboxyl group…

Q: Explain the biochemical defect that leads to Lesch-Nyhan syndrome and suggest how the defect might…

A: Introduction: Purines (adenine and guanine) and pyrimidines (cytosine, thymine, and uracil) play…

Q: Non-standard amino acids are derived from common amino acids by biochemical modifications. Please…

A: All naturally occuring amino acids are chiral except glycine. A chiral center or atom contains…

Q: Why is triose phosphate isomerase considered to be catalytically perfect?

A: Diffusion-limited reactions are reactions where the rate of the reaction is solely dependent upon…

Q: What would be the characteristics of a transitionstate analog for the chymotrypsin reaction?

A: Chymotrypsin : It is a protein enzyme active in the digestive system of many organisms. It…

Q: What amino acids can be found in chymotrypsin’s specificity pocket? What would happen if one of…

A: Chymotrypsin is a proteolytic enzyme.

Q: why is chymotrypsin inactive at high pH?

A: Chymotrypsin is a protease enzyme and thus a digestive enzyme present in the pancreatic juice. It…

Q: Radioactive uracil can be used to label all of the pyrimidine residues in DNA. Using either names or…

A: The nucleotides are classified as purines and pyrimidine. The purines are adenine and guanine. The…

Q: An organic chemist is generally happy with 95% yields. If you synthesized a polypeptide and realized…

A: Introduction: The series of amino acids connected by an amine bond or peptide bonds are…

Q: Please discuss the following statement. "If two ligands involve in the same number and type of…

A: Asked : Statement is true or false

Q: Some of the following four amino acids : alanine, arginine, histidine, aspartic acid would provide a…

A: Introduction: Acid-base catalysis is a mechanism in which the transfer of a proton from an acid…

Q: Using the following data, determine the correct sequence of the octapeptide. Edmund's Reaction =…

A: Chains of Amino acids are held together through a peptide bond to form a structure known as…

Q: TWO of the following statements about Extended Spectrum ß-lactamases are CORRECT?

A: Numerous bacteria produce various enzymes and metabolites that are capable of making the bacteria…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.