Uric acid (UA) is degraded by uricase enzyme immobilized in porous Ca-alginate beads. Experiments conducted with different bead sizes result in the following rate data: 0.6 72 Diameter 0.1 200 Rat hr 196 172 136 112 54 32 a. Determine the effectiveness factor for particle sizes Dp-0.4 cm and Dp 0.6 em b. The following data were obtained for Dp 0.4 cm at different bulk uric acid concentrations. Assuming negligible liquid film resistance, calculate Vmax and Ks for the enzyme. Assume that no substrate or product inhibition. 25 24 50 32 100 40 250 48 UA/ L Rate, r

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