Use the data below to calculate the colony forming units per mL (CFU/mL) from asample of bacteria after growing the sample in an incubator for 2 hours.Culture density (CFU/mL) =Time1 hour2 hours1x 10-¹1 x 10-²1x 10-³1x 104number of colonies(dilution) x (volume plated, in mL)Dilution (before plating) Volume spread on plate (replicate plates)1x 10-11x 10-²1x 10-³1 x 1040.1 mL0.1 mL0.1 mL0.1 mLNumber of colonies observed0.1 mL0.1 mL0.1 mL0.1 mLTNTC6601018TNTC220153TNTC7831157TNTC18097

Serial dilution is the method to calculate the concentration of the micro-organisms in the sample.…

Use the data below to calculate the colony forming units per mL (CFU/mL) from a sample of bacteria after growing the sample in an incubator for 2 hours. Culture density (CFU/mL) = Time 1 hour Dilution (before plating) 1x 10¹¹ 1x 10-² 1x 10-³ 1x 104 2 hours 1x 10-¹ 1x 10² number of colonies (dilution) x (volume plated, in ml) 1x 10³ 1 x 104 Number of colonies observed Volume spread on plate (replicate plates) 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL TNTC 660 101 8 TNTC 220 15 3 TNTC 783 115 7 TNTC 180 26 9 7

Use the data below to calculate the colony forming units per mL (CFU/mL) from a sample of bacteria after growing the sample in an incubator for 2 hours. Culture density (CFU/mL) = Time 1 hour Dilution (before plating) 1x 10¹¹ 1x 10-² 1x 10-³ 1x 104 2 hours 1x 10-¹ 1x 10² number of colonies (dilution) x (volume plated, in ml) 1x 10³ 1 x 104 Number of colonies observed Volume spread on plate (replicate plates) 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL TNTC 660 101 8 TNTC 220 15 3 TNTC 783 115 7 TNTC 180 26 9 7

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200
In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25 colonies the next day. What was the concentration of cells in the original culture? A) 25 cells/ml B) 0.000025 cells/ml C) 25,000 cells/ml D) 26 cells/ml E) 0.025 cells/ml
"If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000
When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?
You perform a quadrant streak and isolate a single colony which contains two distinct species of bacteria. You repeat this with the same results. Provide a possible explanation for this result that does not involve contamination of the sample.
If you counted 300 colonies on a plate that had a 10-5 dilution of bacteria culture, how many colonies might you expect on a plate that had a 10-6 dilution of the same bacterial culture?
How do we reconcile the number of colonies in the plates to the actual number of bacteria in the original sample? Count Plate Method
A bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300. Determine the concentration of cells after EACH dilution. Concentration after the 1st dilution = 6.3 x 107 cells/mL x 1/5 = 12.6 x 106 cells/mL Concentration after the 2nd dilution = 12.6 x 106 cells/mL x 1/300 = 42000 cells/mL
Explain why an isolated colony and a pure culture are not the samething.
A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D C
Below is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asap
Using a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units. If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu? Express your answer in scientific notation rounded to two decimal places. Eg. 0.01001 would be 1.00*10^-2 You must include trailing zeros 0.0000040001 would be reported as 4.00*10^-6 There must always be two decimal places, even if they are zeros Canvas expects one digit before the decimal point 0.40*10^-5 would be marked incorrect for 0.0000040001