Use the equations of the lines plotted in regards to the data pictured to determine Km and Vmax in the absence and presence of the inhibitor. The Km is ____________mM and the apparent Km is  ___________mM. The Vmax is  ______________M/min and the apparent Vmax is  ______________M/min. Therefore, this must be a  _________________ inhibitor.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation
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Problem 30.9QAP
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Use the equations of the lines plotted in regards to the data pictured to determine Km and Vmax in the absence and presence of the inhibitor.

The Km is ____________mM and the apparent Km is  ___________mM.

The Vmax is  ______________M/min and the apparent Vmax is  ______________M/min.

Therefore, this must be a  _________________ inhibitor.

An experiment yields the following kinetic data:
[Substrate] (mM)
Vo (µM/min) no inhibitor
Vo (HM/min) + 1 mM inhibitor
0.2
2.9
1.47
0.3
3.8
1.93
0.4
4.4
2.27
0.5
5.0
2.56
0.6
5.4
2.78
0.7
5.8
2.97
0.8
6.2
3.13
0.9
6.4
3.27
1.0
6.7
3.38
Transcribed Image Text:An experiment yields the following kinetic data: [Substrate] (mM) Vo (µM/min) no inhibitor Vo (HM/min) + 1 mM inhibitor 0.2 2.9 1.47 0.3 3.8 1.93 0.4 4.4 2.27 0.5 5.0 2.56 0.6 5.4 2.78 0.7 5.8 2.97 0.8 6.2 3.13 0.9 6.4 3.27 1.0 6.7 3.38
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