Asked Apr 10, 2019

Voges Proskauer test

Does color of the medium change to red after both reagents were added and allowed to sit for 60 minutes?

If yes, after about how many minutes before you can see red color?


Expert Answer

Step 1

Biochemical method of bacterial identification is a classical method that is used for the characterization of bacteria. These include a set of tests that provides a basic idea about the individual bacterial species in environmental samples such as catalase test, indole test, methyl red test, Voges-Proskauer test, citrate utilization test, oxidase test, urease test, and starch hydrolysis test.

Step 2

Voges Proskauer test or VP test is a test used for acetoin detection in a bacterial broth culture. This test is performed by the addition of alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth inoculated with bacteria. This test is formed to identify whether the bacteria is producing acetyl-methyl carbinol or not from fermentation of glucose.

Step 3

Yes, the color of the medium changes to red color after both reagents were added but it does not allowed to sit for 60 minutes. After inoculation of bacterial broth culture, in a clean test tube broth is mixed with alpha-naphthol and po...

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