We add one drop of each dilution we made from T4rIIAP211 on the left of the plate, and one drop of each dilution we made from T4rIIUV375 on the right site of the plate. Q 10. Draw (or photo) and describe the "AP" and the "UV" spots on the plate. Are the results as expected?
We add one drop of each dilution we made from T4rIIAP211 on the left of the plate, and one drop of each dilution we made from T4rIIUV375 on the right site of the plate. Q 10. Draw (or photo) and describe the "AP" and the "UV" spots on the plate. Are the results as expected?
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 33SST
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Transcribed Image Text:Flow diagram for II. Genetic complementation
Dilute T4rIIAP211
and spot 5 μl:
10-1
10-2
10-4
AP UV
Top agar
E. coli K12(2wt)
T4rIIAP211
Prepare host
lawn plate
Dilute T4rIIUV375
and spot 5 μl:
10-1
10-2
10-4
Transcribed Image Text:We add one drop of each dilution we made from T4rIIAP211 on the left of the
plate, and one drop of each dilution we made from T4rIIUV375 on the right site
of the plate.
10-2
Part II: Genetic Complementation.
Q 10. Draw (or photo) and describe the "AP" and the "UV" spots on the
plate.
Are the results as expected?
-4
10
211
375
E.coli
O
O
10
10-4
Q 11. Occasionally there are plaques in the background in this experiment.
Do you observe such plaques?
What could be possible cause(s) of such plaques?
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