We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c? esday, June 9, 2021, most features of Word will be disabled.ReactivateIn the oxidase test we will determine which bacteria have cytochrome c, one version of the importantenzyme in the electron transport chain,. This enzyme plays a critical role in all those reaction with NADH,however some bacteria use alternative cytochromes. Thus, this becomes another way to distinguishbetween species.Lastly, we will conduct the catalase test. As mentioned in lecture, during respiration some of the hydrogenmay combine with oxygen to form hydrogen peroxide, H2O2. Hydrogen peroxide is lethal to manybacteria. To protect themselves, they produce the enzyme catalase to break it down to water and oxygen.We will determine which bacteria produce this useful enzyme.1. When nitrate is used as the final electron acceptor, what two possible compounds might it be reduced to?2. What reagent is added to culture in nitrate broth to determine if nitrite is present?3. What does a positive test with zinc dust mean for nitrate media?4. We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they containcytochrome c?5. When liquid hydrogen peroxide is dropped on the bacterial colonies for the catalase test, what gas isproduced that causes the bubbles?A Display SettingsFocus

The oxidase test is a commonly used test to identify the presence of cytochrome c oxidase enzyme…

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We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter21: Photosynthesis

Section: Chapter Questions

Problem 5P: The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If...

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Question

We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain cytochrome c?

esday, June 9, 2021, most features of Word will be disabled.
Reactivate
In the oxidase test we will determine which bacteria have cytochrome c, one version of the important
enzyme in the electron transport chain,. This enzyme plays a critical role in all those reaction with NADH,
however some bacteria use alternative cytochromes. Thus, this becomes another way to distinguish
between species.
Lastly, we will conduct the catalase test. As mentioned in lecture, during respiration some of the hydrogen
may combine with oxygen to form hydrogen peroxide, H2O2. Hydrogen peroxide is lethal to many
bacteria. To protect themselves, they produce the enzyme catalase to break it down to water and oxygen.
We will determine which bacteria produce this useful enzyme.
1. When nitrate is used as the final electron acceptor, what two possible compounds might it be reduced to?
2. What reagent is added to culture in nitrate broth to determine if nitrite is present?
3. What does a positive test with zinc dust mean for nitrate media?
4. We will use liquid oxidase reagent for the oxidase test. What color will the colonies turn if they contain
cytochrome c?
5. When liquid hydrogen peroxide is dropped on the bacterial colonies for the catalase test, what gas is
produced that causes the bubbles?
A Display Settings
Focus

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