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Give 2 differences between a DNA library and a cDNA library
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- What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently labeled cDNA.c. known sequences of DNA.d. known cellular proteins.a. If you forgot to add dNTPs to a sequencing reaction, what would be the result? Only very long fragments would be synthesized Only very short fragments would be synthesized The fragments would not be labelled DNA polymerase would be inactivated Sequencing would proceed normally b. Please also answer the image
- List the steps to make a genomic library. What steps would change if you wanted to make a cDNA library instead?Discuss the similarities and differences between RNA polymerase and DNA polymeraseAll of the following are required to create a cDNA library EXCEPT: Group of answer choices DNA polymerase. dNTPs. reverse transcriptase. mRNAs. RNA polymerase.
- Illustrates a Model of Replication Fork Instructions for Making a Model of the Replication Fork1. Color the individual structures on the worksheet as follows:Adenine = red Thymine = greenGuanine = blue Cytosine = yellowPhosphate = brown Deoxyribose = purpleThere are two typical varieties of DNA libraries: genomic and cDNA. How are these made, andwhat are the differences in member cloned DNA fragments.The beta subunits of E.coli DNA polymerase III are responsible for its _______. A. ribosome assembly B. 5’→ 3’ polymerase C. 3’ → 5’ exonuclease and proof-reading D. sliding clamp
- "Complementary DNA (cDNA) libraries offer certain advantages over genomic libraries". Explain how ?Which of the following are unique to RNA polymerase and not found in RDRP? (In other words not shared between the two. Select all that apply.) a) Double-stranded template b) DNA template c) NTP input d) RNA productSanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding: