what are the advantages and disadvantages of using recombinant protein and affinity chromatography for protein purification compared to gel filtration (size exclusion chromatography) and DEAE-sepharose chromatography (ion-exchange chromatography)?

Q: What is replica plating?

A:

Q: is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a…

A: Protein concentration determination is Pa quantitative estimation that can be done in many ways and…

Q: What is the point of hydrolyzing RNA before doing biochemical tests?

A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…

Q: How can I calculate the molecular weight of the unknown protein on this sample using the SDS-PAGE…

A: Electrophoresis of proteins is generally carried out in gels made up of the cross linked polymer…

Q: what is the biochemical basis for affinity purification of a recombinant protein using a 6X his tag…

A: The DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors…

Q: In the Lysozyme purification, if the specific activity in the elate was 108 units/mg and the…

A: Egg white is generally rich in lysozyme and hence it is used for its purification. Lysozyme provides…

Q: Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the…

A: Isoelectric focusing electrophoresis is a technique which is used for separation of amphoteric…

Q: what is the difference between DNA microarray and Fluorescence in situ technique? or is the…

A: Difference between FISH and Microarray Technique Fluorescence In Situ Hybridization It is possible…

Q: Mass spectrometry is a powerful tool in proteomics. What are the four key features of a mass…

A: The mass spectrometer converts the individual molecules or compounds into ions such that they become…

Q: Briefly describe the importance of two dimensional electrophoresis in protein separation?

A: Two-dimensional gel electrophoresis is the technique that is used in the separation of the proteins…

Q: When the gel is removed from the chamber in SDS-PAGE, only the “pre-stained” protein standard is…

A: SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is commonly used as a method…

Q: What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: What is transformation efficiency and how is it calculated?

A: Transformants are the cells that are able to incorporate any modified foreign or artificial DNA.…

Q: What reaction would you expect when performing a positive control in the catalase assay? What would…

A: A catalase assay or test is implemented to differentiate streptococcus bacteria from staphylococcus…

Q: How many DNA bands would you observe in an ethidium bromide-stained electrophoresis gel if you…

A: In agarose gels and various cesium chloride gra- dient techniques, ethidium bromide is employed to…

Q: At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How…

A: Plasmid training is a way of DNA extraction and purification for plasmid DNA. Many techniques were…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: Can two-dimensional gel electrophoresis be used as a purificationtechnique? Explain.

A: Gel electrophoresis is a laboratory technique, which is used to separate mixtures of DNA or RNA…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: In the alkaline plasmid screen, what two things separate plasmid from chromosomal DNAs?

A: Following is the procedure for the separation of plasmid by the alkaline lysis method.

Q: In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?

A: Urea assay (catalogue #K375-100) is a colorimetric assay used to quantify the urea in any given…

Q: t is the principle of gel electrophoresis?

A: The purity of isolated nucleic acids is determined using electrophoresis.

Q: In a protocol for DNA sample preparation for agarose gel electrophoresis, what volume of 4X loading…

A: Electrophoresis  It is a biochemical technique to separate charged molecules under the influence of…

Q: Give three reasons why liposomes/nanoparticles are attractive drug delivery system for recombinant…

A: Introduction: Liposomes are a small-sized vesicle sharing structural and compositional similarity…

Q: What are the different applications of Gel Electrophoresis?

A: The variation in size and charge of distinct molecules in a sample is exploited by the gel…

Q: Discuss the underlying biochemical principle of the nucleic acid sequencing methods known as…

A: One of the techniques for figuring out the sequence of DNA or RNA is called next-generation…

Q: Using Bradford Assay, plot the standard curve and find the unknowns. Protein Concentration A595 of…

A: Standard curve  A set of standards are put up in the Bradford assay procedure from protein sources…

Q: What is the purpose of a standard curve for a colorimetric enzyme assay?

A: Enzyme assays are mostly used to determine the vitamin status in the body. The activity of…

Q: What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle…

A: The purpose of Southern blotting technique is  to identify specific DNA from the mixture of other…

Q: s in protein assays? Explain briefly.

A: Protein assays can be utilized as the methods life science research in order to  estimation of…

Q: Electrophoresis is an extremely useful procedure when applied to analysis of nucleic acids as it can…

A: Gel electrophoresis is a technique that separates the macromolecules based on their mass and charge.…

Q: fragments will be formed-a, b, and c. Which of the following gels produced by electrophoresis would…

A: A DNA segment is cut by two restriction enzymes and the three fragments are separated on an agarose…

Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: what are two differences between agarose gel electrophoresis and SDS PAGE?

A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…

Q: In the isolation of DNA which of the following best describe the purpose of using NaCl-SDS solution?

A: DNA extraction is a technique for separating DNA from cell membranes, proteins, and other cellular…

Q: When Avery and his colleagues had obtained what was concluded to be the transforming factor from the…

A: Proteases, also called peptidases, are the enzymes that catalyze the breakdown/lysis of proteins…

Q: How is the Bicinchoninic acid assay used in protein quantification?

A: The Bicinchoninic Acid Assay (BCA), often known as the Smiths' Assay, was developed by Paul Smith in…

Q: Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay…

A: This assay is used to measure the concentration of protein.

Q: What volume of 4X loading buffer must be added to 21 micro L of DNA in a technique for DNA sample…

A: Electrophoresis is the technique of seperation of RNA DNA and protein molecules on the basis of…

Q: what is isosterism and bioisosterism? how does this concept help in biotransformation of different…

A: The term Pharmacokinetics is used to refers to the measurement of the fate of a pharmacological…

Q: how does polyacrylamide gel electrophoresis with SDS separate proteins ?

A: Answer: SDS = Sodium Dodecyl Sulfate is a detergent used in the polyacrylamide gel electrophoresis…

Q: How can Size Exclusion Chromatography be used to monitor heat-induced changes in whey proteins?

A: Size exclusion chromatography is a type of column chromatography where the column is packed with…

Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification…

A: Protein purification is a process of isolation of specific protein from the complex protein mixture…

Proteins

We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.  

Protein Expression

The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.