What are types of cell culture media?

Q: What are the materials used to grow microorganism like Spirulina?

A: Introduction In this question we will discuss about the materials used to grow microorganisms like…

Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…

A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…

Q: What is agar and why is it necessary for growing bacteria on plates?

A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…

Q: What is the best growth medium that contains glucose that can be used to acclimatize bacteria? and…

A: A growth medium is a liquid, solid, or semi-solid preparation that supports the growth of a…

Q: How does the heaviness of a bacterial smear affect its microscopic analysis?

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?

A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…

Q: Given a log phase bacterial culture with 1 x 10^6  cells per ml and a generation time of 30 minutes…

A: Cell multiplication is due to the growth which leads to an increase in the number of bacteria over a…

Q: How does the plant cell wall react when it is placed under hypotonic medium?

A: The plants are the multicellular eukaryotic organism of the kingdom Plantae. It is the autotrophic…

Q: How to distinguish bacteria if a bacterial colony formation happens and a certain bacteria appearing…

A: Bacteria flourish on culture media in the form of colonies. A colony is specified as a visible mass…

Q: How are biofilms significant to the human body and the environment?

A: Significance of biofilms:                     A vital structures associated with microorganisms…

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: What is secondary cell culture?

A: Secondary culture is a subculture of the primary culture. All the cells grown in the Petri dish…

Q: How are  cells  formed in de novo from aboitic materials?

A: A cell is a structural and functional unit of life. The structure and function of a cell have been…

Q: CTSA growth medium designed

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: What is differential medium and differential media? Explain the difference ?

A: Difference between differential medumium -   .- A differential medium support the expansion of any…

Q: What external bacterial cell structures can be demonstrated by a negative stain?

A: The negative stain is useful in studying the morphology of bacterial cells. It also characterize…

Q: What does the Gram stain reveal about a cell?

A: Gram stain is used for the primary identification of the bacterial cells. In this test, violet dye…

Q: A growth medium consisting of only known amounts of water, magnesium, calcium chloride, potassium…

A: The growth medium, also called as Nutrient Broth, is a kind of solution which is sterilized to get a…

Q: Why would you want to limit the number of times you passage a cell culture line?

A: Cell culture is the process through which cells are cultivated under controlled conditions,…

Q: what is the culture media for gram-positive bacteria?

A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: describe the macro and microenvironments within a cell culture laboratory.

A: The factors that are in the immediate vicinity of the microorganism and thereby have a direct &…

Q: What are the kinds of microtome with their corresponding type of embedding medium?

A: A microtome is a cutting tool that creates incredibly tiny material slices called sections.…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell organelles extraction  The process to obtain cell organelles from the cell.

Q: ll might appear Gram negative?

A: Bacteria can be defined as small single-celled organisms. Bacteria are present almost everywhere on…

Q: What prevents Elodea cells from becoming shriveled up like a blood cell would become if placed in a…

A: Hypersonic solutions are those which have high concentration of salt in it.

Q: What is the order of staining in microbiology

A: Crystal Violet - Primary stain ( Stain the cell wall  of the microorganisms)  Gram's iodine - Acts…

Q: why the Gram stain can differentiate between Gram positive and Gramnegative cells?

A: *Gram staining is a technique used to differentiate two groups of bacteria into gram positive and…

Q: How should agar plates be incubated? Why?

A: Incubating the plates to stimulate the growth of microbes is a crucial step in any microbiology…

Q: What technology is available for faster bacterial culture?

A: BASIC INFORMATION BACTERIA  It is one of the type of biological cell. They belong to prokaryotic…

Q: What is the shape of bacteria under a microscope, what color is there, and is there Gram staining is…

A: Introduction- Gram staining is basic staining to identify the bacteria of medical significance. Gram…

Q: How is household antibacterial soap like a weapon against pathogenic bacteria?

A: Antibacterial soaps are those that contains certain chemicals that inhibit the growth of pathogens .

Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…

A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…

Q: What type of agar is MacConkey agar?

A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…

Q: What is gram negative and gram positive?

A: Gram staining is the type of staining to determine if the microorganism of interest is gram negative…

Q: What factors determine the eventual size of bacterial colony?

A: Bacteria grow on solid media as colonies. A colony may be defined as a visible mass of…

Q: Why are agar plates incubated upside down?

A: The Petri dish has existed for over a decade, whereas simple, cell-growing equipment remains…

Q: What are the possible contamination in animal cell tissue culture laboratory and list important…

A: Animal cell culture is a technique in which the cells are grown obtained from animals are grown…

Q: On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why…

A: Question - On agar plate does each discrete colony represent the growth of one cell? Explain your…

Q: explain Bacterial cultures.

A: Bacterial culture is a microbial test to determine the type of organism, its abundance in the sample…

Q: What is a microbial colony and how is one formed?

A: Bacteria are a prokaryotic microbe. Most of the bacteria’s are pathogenic and parasitic living in…

Q: How do the differences between gram-positive and gram-negative cell walls influence the cell’s…

A: Bacteria are minuscule, single-celled living beings that flourish in assorted conditions. These…

Q: Write the limitations of sterilization by Dry heat?

A: The dry heat sterilization technique includes the removal or killing the microorganism and require…

Q: How do the chemicals such as aldehydes, gaseous sterilizers, peroxygens, and organic acids affect…

A: Physical and chemical methods are used to control the microbes. Chemical methods are where chemicals…

Q: Which piece of equipment sterilizes hospital tools and devices and how does it sterilize things? Are…

A: Sterilizing pieces of equipment are very important in modern healthcare settings. It is necessary…

Q: What are the differences between “primary cell culture” and “continuous cell lines”?

A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.