Question
Asked Oct 5, 2019
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What does the pink band in a FTM indicate?

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Expert Answer

Step 1

Thioglycolate broth is an enriched, and differential medium, which is used to determine the requirement of oxygen by microorganisms. Sodium thioglycolate is present in the medium, which consumes oxygen and allows the growth of obligate anaerobes. This results in the diffusion of oxygen from the top and producing a range of oxygen concentrations in the medium.  

Step 2

Fluid thioglycolate medium (FTM) is used for the cultivation of anaerobes, aerobes, and microaerophiles. This medium contains dextrose, yeast extract, tryptone, and L-cystine for bacterial growth. Apart from these, sodium thioglycolate and agar are also present to favor the growth of aerobes and anaerobes.

In FTM, the microorganisms arrange themselves in a sequence depending on their oxygen requirement. For...

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