
EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN: 9781305446021
Author: Lampman
Publisher: CENGAGE LEARNING - CONSIGNMENT
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What error is introduced into the determination of an Rf value if the top is left off the developing chamber?
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Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.
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what will produce a smaller Rf value if the solvent and paper are both changed?
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At the completion of the experiment, a student measures the solvent front to be 55 mm from the baseline. If a component in the sample being analyzed generates a spot whose diameter spans from 34 to 36 mm from the baseline, calculate the Rf value for that component.
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a pigment in the thin layer chromatography traveled beyond or farther than the solvent front. Therefore, the Rf value of that certain pigment is greater than 1.Ā
What does this mean to the experiment?
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if air can degrade a column and cause TC detector filaments to burn out, why do we use an air peak?
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What is the Rf value for a compound if it moves 3.0 cm from the origin when the initial spot is developed and the solvent front is 12.0 cm from the origin?
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If a spot travels 2.3 cm while the mobile phase travels 6.6 cm, what is the Rf value of the substance in the spot? What is the mobile phase used and stationary phase used in this lab?
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It's important to mark the solvent front with a pencil after the plate is finished being developed in order to calculate an Rf.
Group of answer choices
True
False
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Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the sensor increases? Explain
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Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move 9.1 cm from the origin
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Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the following in your drawing:
Solvent front
Origin
Mobile phase
Stationary phase
Distances needed to measure Rf value for one of the spots
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Explain why it is needed to filter the solvent before introducing it to the HPLC system
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