What happens if the spots are made too small when preparing a TLC plate for development?

Q: Why are some of the detectors listed unsuitable for HPLC?

A: HPLC  is applicable for the use with solvent that has UV absorbance. A UV detector cannot be used…

Q: How do i know my starting material was fully consumed using a TLC plate comparing the raction…

A: TLC means thin layer chromatography is a chromatographic technique used for the separation of…

Q: d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the…

A: In this question, we have to find out the correct answer of given problem by the help of the concept…

Q: In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging…

A: High-performance liquid chromatography (HPLC) is one of the separation technique used to analyze of…

Q: Explain how the Rf (Retention Factor)-values can be in agreement or disagreement with the “like…

A: Interpretation -  To explain all about that how the Rf ( Retention factor ) - values can be…

Q: What is the importance of paper chromotography?  What is the usage of paper chromotography in the…

A: Paper chromatography is a chromatography technique that is used for separating colored pigments…

Q: What is the benefit of using fluted filter paper instead of not folding it?

A: “Fluted “ filter paper (LEFT) is used when we wish to separate a liquid and a solid, keeping the…

Q: A student ran a TLC plate using a 90:10 hexane:ethyl acetate developer and this was the outcome. How…

A: Given that , A student ran a TLC plate with 90:10 of n-hexane : Ethyl acetate.

Q: What happens if the spots are made too large when preparing a TLC plate for development?

A: This question is related to chromatography.

Q: magine you were separating acetophenone from hydroquinone. Would you expect acetophenone to be the…

A: We know Acetophenone is less polar than hydroquinone, as a result, acetophenone would be the top…

Q: solvent front origin

A: In thin layer chromatography the compounds separate based on polarity, the more polar compound move…

Q: What is chiral chromatography?

A:

Q: One of the following techniques cannot be used for separation ounodwom Select one: O a.…

A: Answer - The correct option is (b) boiling point determination. The boiling point of a compound,…

Q: Why should you not use a ballpoint pen when marking a TLC plate and why does the horizontal line…

A: If ball point pen is used, the ink will travel up with the solvent, just like the samples.  The…

Q: What are the major differences between GC and HPLC, discus the types of samples among other…

A: GC stands for "Gas Chromatography" whereas HPLC stands for "high performance liquid Chromatography"…

Q: TLC.

A: TLC  Work by polarity of compounds. that means high polarity of a compound then lowest distance…

Q: Your lab-mate observes a large amount of streaking and blurring of their samples when they visualize…

A: It is the major problem that YTLC plate seems to be blurred in the UV light.  But you can overcome…

Q: What could happen if you used ink to draw on your baseline and letters on the TLC plate?

A: Pencil is always used to mark chromatography paper.

Q: Name 3 adsorbents used in TLC. Mention their use and properties.  Why is it important to use a…

A: 3 adsorbents used in TLC -  1. Silica gel : Silica gel is by far the most widely used adsorbent and…

Q: Why is it important to handle the TLC plate in such a way that your fingers do not come in contact…

A: Chromatography works on the principle that different compounds will have different solubilities and…

Q: look for at least three extraction methods for perfumery materials.

A: There are several methods which are used for extraction of the perfumery materials from different…

Q: If a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could…

A: Rf value Vs polarity of the eluent

Q: Which statement about TLC analyses is not true? *       A- The line at the base of the TLC plate…

A: TLC is thin layer chromatography.  It is a chromatographic technique to separate the components of…

Q: Fill in the blanks: is applied when TLC will be used to isolate compounds. It uses a plate.

A: Thin layer chromatography is a technique used to isolate non volatile mixtures.

Q: You can perform a TLC experiment even if the solvent level is above the level of the initial spots.…

A: Here I have solved the question

Q: Would the spots on the TLC plate in Scenario 3 be visible under UV? Why or why not?

A: Thin layer chromatography is used to separate the non-volatile substances from a mixture. Here, two…

Q: how might chromatography be used to analyze the composition of the dye in a marker?

A: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which…

Q: At what step in the recrystallization process would a colored impurity be removed?

A:

Q: What are the two types of membrane usually employed for western blotting?

A: The two types of Membrane employed for western blotting are:- (1) nitrocellulose  (2) PVDF…

Q: Many ink dyes can be separated by the technique of___________ chromatography

A: Thin Layer Chromatography is a method for separating non-volatile substances. The separation…

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: describe different type of degasser in HPLC system, and why degassing is important in the middle of…

A: Degasser is  very useful in HPLC .the main use of degasser in HPLC are  hellium sparging, sonication…

Q: What is the purpose of evaporating off a majority of the pentane solution before spotting the TLC…

A: In thin-layer chromatography, TLC, in order to see the spot, visualization techniques are used in…

Q: What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to…

A: TLC works on the basic principle of separation of compounds based on the interaction of the sample…

Q: You will run a TLC plate after the CC placing in one spot the original sample of pigments, one spot…

A: Thin Layer chromatography (TLC) TLC is a chromatographic technique used to separate mixtures of…

Q: After acid-base extraction why is it important to wash organic layer at least once with pure water?

A: A question based on quantitative analysis, which is to be accomplished.

Q: how to know if my product was pure usiing a TLC plate that has reaction mixture, co spot, and…

A: Thin layer Chromatography is also known as TLC which is a very useful tool to check a reaction is…

Q: What happen to the glowing wood splint when it is inserted inside the test tube?

A: Given,          Glowing wood splint is inserted into a test tube     Required,…

Q: Which might you need to do a ninhydrin stain to visualize spots in TLC?

A: The solutions used in TLC to detect whether a reaction is taking place or not might not have colour…

Q: improve

A: We know that resolution is a measure of how well two Elution peak can be differentiated in…

Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system

A: Basically there are following two reasons for using filtration during HPLC sample preparation are…

Q: A) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC…

A:

Q: how does TLC determine if a sample is pure?

A: checking for purity of a sample,  the known sample which is pure(having only one spot) and has the…

Q: What kind of chromatography would you for purification of full length antibody ?

A: Chromatography is used to seperate and purify mixture of species . Various type of chromatrographic…

Q: Identification of spots on the TLC plate is done by all of the following EXCEPT O Spraying with…

A: TLC is thin layer chromatography used to know the components in mixture and generally used to check…

Q: 1. Which property of compounds is responsible for the separation in a TLC? Explain with an example.…

A: Please find your solution below : 1. Thin layer chromatography is a technique used to separate the…

Q: When would using Thin Layer Chromotography (TLC) NOT be useful in conducting an oxidation reaction?…

A: Although it is a very simple and convenient technique, one of its limitations is that it cannot tell…