What is a PCR?

Q: How does PCR relate to biomedical engineering?

A: Introduction Biomedical engineering is a branch of engineering concerned with biology and medicine.…

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: why different thermal conditions are necessary for successful PCR?

A: DNA polymerase is an enzyme that naturally polymerizes nucleotides to polynucleotide strands. The…

Q: Why is MgCl2 added to the PCR reaction?

A: De-novo gene synthesis is becoming an important tool for recombinant DNA technology. The Denovo gene…

Q: Define the term PCR.

A: Dr.Kary Mullis developed the polymerase chain reaction (PCR) in 1983. The enzyme utilised in this…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: How do you interpret PCR?

A: Polymerase chain reaction is method of obtaining large quantities of DNA sequence by selective…

Q: what are the variants of PCR ?

A: PCR ( polymerase chain reaction) is a test tube method for amplifying a selected DNA sequence. There…

Q: How does PCR work, and why is it useful?``

A: Polymerase chain reaction (PCR) is a molecular biology technique to generate numerous DNA strands…

Q: Why is PCR beneficial?

A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…

Q: After four cycles of PCR, which products predominate? Explain why.

A: Polymerase chain reaction (PCR) is a type of genetic analysis in which the nucleotide sequence of…

Q: How do you know if it works? If you have more than one band in a lane, why what would be the reason

A: PCR is a polymerase chain reaction and it is used to amplify the segment of DNA/Nucleic acid.

Q: What is Proteomics ?

A: Proteins are naturally occurring substances that are highly complex and made out of amino acids that…

Q: What different techniques can be used for transcriptomeanalysis ?

A: The transcriptome is the set of all RNA transcripts including coding and non-coding in an individual…

Q: Why is extra primers added to the pcr reaction?

A: Introduction :- The polymerase chain reaction, or PCR, is a chemical reaction used by molecular…

Q: In the second step of the PCR, the temperature is reduced to between 45-65 degrees C. What happens…

A: Polymerase chain reaction (PCR) is considered a molecular technique to produce multiple copies of a…

Q: What is linear amplification PCR?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: Name four reagents used during a PCR reaction

A: Polymerase chain reaction: It is an in vitro technique that results in the exponential…

Q: How many DNA molecules would exist after to PCR cycles?

A: The polymerase chain reaction is a technique that is widely used in disease diagnosis, forensic, DNA…

Q: What are the criteria for “good” primers in a PCR reaction?

A: Polymerase chain reaction (PCR) is the method that is widely used to make billions of copies of a…

Q: What is genome sequencing?

A: A genome is defined as a complete set of DNA in an organism which is also known as the blueprint of…

Q: What is the first step that should be done in PCR testing?

A: PCR is the polymerase chain reaction. PCR test or polymerase chain reaction test is good method to…

Q: Why  do the two possible PCR products differ in size by  300 base  pairs?

A: In in vitro conditions, a polymerase chain reaction (PCR) is a technique for making several copies…

Q: What is PCR? How does PCRworks?

A: The polymerase chain reaction ( PCR) is also called "molecular photocopying" and is a quick and…

Q: What are the steps in shotgun sequencing?

A: Shotgun sequencing is a laboratory method which is used to determine the sequence of DNA of an…

Q: What is a read (Sanger sequencing)?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: Why is it important to wear gloves when setting up the PCR tubes?

A: PCR represents polymerase chain reaction which is an in vitro laboratory technique used for the…

Q: What are the three steps of PCR?

A: PCR (polymerase chain reaction) is a technique, used for the in vitro amplifying desired DNA…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: What is clinical exome test?

A: The exome is the part of the genome which contain all the protein coding sequences.

Q: If the PCR negative was positive what would that mean?   If the PCR positive was negative what…

A: PCR is also known as polymerase chain reaction is used to amplify the DNA and get accurate results…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: When is the transformation factor used?

A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…

Q: What is the final volume of the individual PCR reactions we are making?

A: Polymerase chain reaction (PCR) is an in vitro method of making large number of copies of desired…

Q: What is next generation sequencing technology?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: Who created the gene gun?

A: Any of the various substances that are generated by living organisms and cells are called…

Q: What is a Sanger Sequencing ?

A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…

Q: What are the reagents and equipment used in PCR?

A: Polymerase Chain Reaction(PCR) is a laboratory technique introduced by Kary Mullis in 1980s to…

Q: In PCR, the goal is to make copies of?

A: The field of biology concerned with the molecular foundation of biological activity in and between…

Q: In the third step of the PCR, the temperature is raised to 73 degrees C.  What happens during this…

A: PCR: PCR (polymerase chain reaction) is a technique used to make multiple copies of a particular…

Q: What is the purpose of the PCR?

A: PCR is a technique which is used for the amplification of specific segments of DNA from a larger DNA…

Q: What is a transgenic organism?

A: Recombinant DNA technology involves integrating DNA molecules from two different species and…

Q: What determines the size (length) of the primary PCR product?  What might a successful gel check of…

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene of…

Q: What are the advantages of PCR?

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…

Q: What was the purpose for doing the colony PCR?

A: Colony PCR is a method done to screen the colonies of yeast or bacteria that have grown with the…

Q: at is the purpose of the PCR technique in Covid testing?

A: The question is all about the PCR technique i.e polymerase chain reaction technique. It is the…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.