What is meant by the term plating efficiency?

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: Why does the oxidase test have to be read quickly?

A: The Oxidase test is a biochemical test for the identification of bacteria. The test is mainly…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: The two most abundant pigments in the chromatography results were carotene and Xanthophyll. What…

A: A photosynthetic pigment is a pigment which is found in green plants and do not  absorb all the…

Q: where will you dispose of the waste from Molisch test?

A: Sugars or carbohydrates are the most abundant biopolymers in living organisms. Carbohydrates are…

Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…

A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…

Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…

A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…

Q: Why is the annealing temperature different between samples? How is this computed?

A: Introduction Polymerase chain reaction or PCR  laboratory technique by which millions to billions of…

Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…

A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).

A: Column chromatography is one of the most useful methods for the separation and purification of both…

Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…

A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…

Q: In the determination of crude fiber, why should the sample used be finely grounds and exhausted with…

A: The crude fiber found in our food which is an insoluble carbohydrate remains insoluble in our body…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM…

A: Volume of cell culture = 1.5 ml = 1500 microL(V2) (1 ml = 1000 microL) Desired colchicine…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: By the means of gas stoichiometry, how soaps are created?

A: Soap is made up of animal fat or plant oil, as well as caustic soda. It dislodges dirt from surfaces…

Q: Why is immobilization a good strategy for dealing with uraniumpollution?

A: Uranium (U) is a radioactive chemical element of the actinoid series of the periodic number with an…

Q: How did the Hershey–Chase “blender experiments” confirmGriffith’s results?

A: Based on the Griffith experiment's observations, he concluded that genetic material is the…

Q: What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its…

A: Triglycerides are found in fats and oils. Fatty acyl esters join three fatty acids to glycerol to…

Q: What is the correlation between radiation “energy” and the mechanisms of sterilization?

A: Safety is of utmost importance while working with any contagious or pathogenic substance or living…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: What are the two principal types of mass spectrometry?

A: Mass spectrometry is used to determine the mass-to-charge ratio of ions and separate them according…

Q: What are two types of compounds that make up the resin for column chromatography?

A: Column chromatography is a technique used for the separation or purification of biomolecules such as…

Q: How would you prepare 10 to the power -4 dilution from a culture?

A: Bacterial culture or generally microbial culture is a method of multiplying the microorganism by…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: What is one advantage of a pour plate over a streak plate?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: How do I find the optimal temperature for the iodine and starch experiment?

A: The optimal temperature for a particular enzyme is the temperature at which the enzyme attains…

Q: Wintrobe from Westergreen regarding accuracy of the test in a tabulated order.

A: Both methods are used for estimating E.S.R rate or the erythrocyte sedimentation rate. Most of the…

Q: When preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?

A: Electrophoresis is a laboratory technique used to separate and isolate desired nucleic acid…

Q: For microprojectile bombardment, the particles used are typically? a. Nickel b. Tungsten O c.…

A: Ttransformation is a biotechnological process where a foreign DNA molecule is inserted into the host…

Q: What is meant by the term “enrichment culture”?

A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…

Q: Why do we add powdered zinc to the nitrate reduction test?

A: Bacteria are prokaryotic simple unicellular organisms. Their mode of respiration may be aerobic or…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: What is the meaning of BSC or biological safety cabinet and why is it important and how is it works?

A: Introduction: Biosafety issues refer to the procedures, policies, and principles to be adopted to…

Q: Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?

A: INFILTRATION is the process in which the clearing agent is completely removed from the tissue and…

Q: What is the difference between copay, coinsurance, and deductibles?

A: The inpatient refers to client who is admitted to the hospital for the treatment of his/her illness…

Q: what color will cellulose turn for iodine test?

A: Cellulose is an important component of cell wall of plants. It is a Polysaccharide made up of beta D…

Q: How will you know when the endpoint of the titration is reached? Why is it necessary to mix the…

A: Reaction  It is a process in which reactant molecules react to each other and form another molecule…

Q: Why is cobalt chloride paper used in the experiments?

A: The indicators are used in the experiment to detect the distinct observable change. Many types of…

Q: What two procedures should you routinely carry out to verify the purity of your working stock?

A: The 2 procedures are shown below.

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: What is the basic purpose of the paper chromatography?

A: Chromatography is an important analytical method used to separate a mixture of chemical substances…