Question
Asked Nov 12, 2019
1 views

What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?

check_circle

Expert Answer

Step 1

Your question contains multiple sub-parts, and as per our policy, we only answer the first three subparts. So, if you want the answer of remaining parts please post separately.

Step 2

Sanger sequencing – Developed by Frederick Sanger is a method by which the sequence of DNA can be determined. It is done by incorporating chain-terminating dideoxynucleotide triphosphates (ddNTPs) by DNA polymerase in in-vitro conditions.

The Sanger dideoxy method of DNA sequencing requires a single-stranded DNA template, a DNA primer, a DNA polymerase, normal deoxynucleotide triphosphates (dNTPs), and modified di-deoxynucleotide triphosphates (ddNTPs).

Step 3

Modified di-deoxynucleotide triphosphates (ddNTPs) are used along with the normal deoxynucleotide triphosphates (dNTPs)-  it is because the modified ddNTPs terminate DNA strand elongation. These chain-terminating nucleotides lack a 3'-OH group required for the formation of a&nbs...

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour.*

See Solution
*Response times may vary by subject and question.
Tagged in

Science

Biology

Molecular Biology

Related Biology Q&A

Find answers to questions asked by student like you

Show more Q&A add
question_answer

Q: How Big Should Each Offspring Be? Can you please use examples for the reading,

A: According to Elgar and Berrigan, there is a negative correlation between size of offspring and clutc...

question_answer

Q: List mechanisms for control of microbial organisms for physical, chemical, and mechanical methods.

A: The growth of microorganisms can be controlled in two ways: either by killing the organism or by con...

question_answer

Q: What chromosome rearrangement is thought to be the source of new genes?

A: Chromosome rearrangement is a change in the order of base sequences along one or more chromosomes. I...

question_answer

Q: List the characteristics of a local potential versus an action potentials.  Describe where each happ...

A: Characteristics of a Local potential versus an Action potential are given below in the table:

question_answer

Q: Estrogens stimulate the development of ______. sperm production facial hair a low-pitched voi...

A: The estrogen is the major female sex hormone and it is responsible for the regulation and developmen...

question_answer

Q: If we extract DNA of a pool of cells, will that impact the DNA sequencing? In other words, having mo...

A: Since we only answer one question at a time according to our honor code, therefore we’ll answer firs...

question_answer

Q: What prevent the lungs and chest wall move inward during expiration

A: Expiration is the flow of the breath out of an organism. It is the movement of air from the lungs ou...

question_answer

Q: list other biological examples in which specific (bacteriophage specificity) protein interactions ar...

A: Protein-protein interaction (PPI) is the physical contact or the interaction established between two...

question_answer

Q: If the genetic code were overlapping, how many complete codons would the followingsequence encode be...

A: The process by which mRNA is translated to the amino acid sequence is termed as translation. It occu...