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A: In the first step their is answer of first four molecules as follows:
What is the method of MS protein quantification presented here?
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- Which peptide would absorb the most UV light at 280nm? (Can you show work because I mainly want to know how to solve this type of problem. Thank you!)Leu-Trp-Tyr-Ala Tyr-Lys-Tyr-Cys Glu-Tyr-Ile-Arg Ala-Trp-Trp-Ala Thr-Ala-Ile-ThrHydropathy plot analysis of your protein of interest reveals a single, prominent hydrophobic peak. However, you later discover that this protein is soluble and not membrane associated. Explain how the hydropathy plot may have been misleading.Vasopressin: CYFQNCPRG Oxytocin: CYIQNCPLG Calculate the pI of each peptide. How could the differences affect their function.
- A protein was purified and then analyzed on a size exclusion column. It's molecular weight was determined to be 40,000 Dalton. The SDS-PAGE gel shows one band, corresponding to a molecular weight og 10,000 Dalton. Describe the native conformation of this protein in terms of the number of subunits present and the molecular weight of the subunits.A protein was purified and then analyzed on a size exclusion column. Its MW was determined to be 50,000 Dalton. The SDS-PAGE gel shows two bands, corresponding to molecular weights of 10,000 and 15,000 Dalton. Describe the native conformation of this protein in terms of the number of subunits present and their molecular weight.A protein-ligand binding reaction is run. At equilibrium, half the protein is ligand bound, the unboundligand concentration is 0.657 nM. Calculate the koff value for this reaction. Assume the kon value is typical ofprotein-ligand interactions.
- What is the difference between Lamin A protein and progerin? briefly describe the difference in their functions ( three sentences or less) Flippases help maintain membrane asymmetry. True FalseProtein A will fold into its native state only when protein B is also present in the solution. However, protein B can fold itself into native confirmation without the presence of protein A. Which of the following is true? a. Protein B serves as precursor for protein A b. Protein B serves as molecular chaperone for protein A c. Protein B serves as ligand for protein A d. Protein B serves as structural motif for protein A
- don't copy 6. A protein-ligand binding reaction is run. At equilibrium, half the protein is ligand bound, the unboundligand concentration is 0.657 nM. Calculate the koff value for this reaction. Assume the kon value is typical ofprotein-ligand interactions.The movements of single motor-protein moleculescan be analyzed directly. Using polarized laser light, it ispossible to create interference patterns that exert a cen-trally directed force, ranging from zero at the center to afew piconewtons at the periphery (about 200 nm from thecenter). Individual molecules that enter the interferencepattern are rapidly pushed to the center, allowing them tobe captured and moved at the experimenter’s discretion.Using such “optical tweezers,” single kinesin mol-ecules can be positioned on a microtubule that is fixed toa coverslip. Although a single kinesin molecule cannotbe seen optically, it can be tagged with a silica bead andtracked indirectly by following the bead (Figure Q16–3A).In the absence of ATP, the kinesin molecule remains at thecenter of the interference pattern, but with ATP it movestoward the plus end of the microtubule. As kinesin movesalong the microtubule, it encounters the force of the inter-ference pattern, which simulates the load…Mild, non-ionic detergents (like Triton X-100, with polar but uncharged regions that do not denatureproteins) would be required for separation of which of the following proteins from cell membranes?A. monolayer-associated proteinsB. lipid-linked proteinsC. transmembrane proteinsD. integral proteinsE. peripheral proteins