Q: Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0…
A: Please follow step 2 & 3 for detailed explanation.
Q: 1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2.…
A: the Kato-Katz approach is used for qualitative and quantitative diagnosis of intestinal helminthic…
Q: 1. Provided with the following data, compute the corresponding CFU/ml of the original culture.…
A: Various bacterial species are responsible for a variety of diseases in humans. As a result, we apply…
Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…
A: A dichotomous key is a tool designed by scientists to determine the identity of the organism. Here,…
Q: Why must m-Endo broth or some similarly selective and differential medium be used to count…
A: Coliform bacteria are described as rod-shaped, Gram-negative, nonspore-forming, motile or nonmotile…
Q: 1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2)…
A: Hi! Thanks for your question. As you have posted multiple questions, I am answering only the first…
Q: 2. The following is an incomplete schematic diagram for the isolation procedure used. Label each…
A: Isolation of Micro organisms -- Introduction --The term isolation is defined as separation of a…
Q: lly Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a…
A: CFU is an abbreviation for Colony Forming Unit. It is used to calculate the number of viable…
Q: Why do you disinfect your work area at the beginning of lab and then again at the end of lab? 2.…
A: Pure culture - In microbiology if one organisms cultured in midium. It is obtained from mixed…
Q: 1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame?…
A: “Since you have posted a question with multiple sub-parts, we will solve first three sub parts for…
Q: Urinary Tract Infection
A: 1. Critical threshold refers to transition range when small change in spatial pattern produces…
Q: 1. List 5 importance of using selected and differential culture media. 2. Mannitol Salt Agar is a…
A: 1. Importance of using selective and differential media Selective and differential media are used to…
Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…
A: The question asks about the test that can be used on the disease-causing organism below that can…
Q: 2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A.…
A: Hello, thank you for your question. As per our policy, I am going to answer only the first question.…
Q: b. Successfully completed most steps of the Gram stain but failed to add the iodine (the mordant):…
A: Gram staining is a differential staining procedure used to classify two types of bacteria on the…
Q: what is plaque essay? what is the purpose of this lab? why this lab is perform? (bacteriophage) what…
A: In plaque essay, the bacterial cells are added into the medium during log-phase. Due to this, a…
Q: 1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the…
A: Given, each time 100 ul is added into 400 ul of diluent solution. Also,100 ul is added into culture…
Q: Possible Unknown Strains: Stapylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus,…
A: INTRODUCTION Not only are most bacteria very small, they're also very clear and difficult to look…
Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…
A: PREPARATION OF SMEAR ( FLOWCHART)
Q: are there technologies that can help make bacterial culture and sensitivity done faster
A: Bacteria culture is the method to use for the analysis of the amount of infection caused by…
Q: 1. The differential aspect of MacConkey agar allows for the determination of which bacteria are…
A: selective media allow a certain type of bacteria to grow while inhibits the growth of other…
Q: 9. Complete the table by transcribing the following culture media, classify as to…
A: Introduction : A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: Give the importance of the following factors in bacterial characterization methods: (a) cultures,…
A: A microbe is a living entity that is so tiny that it cannot be seen with the naked eye. Microbiology…
Q: What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What…
A: Denitrification - It is the process by which nitrate (NO3-) is reduced to produce nitrogen (N2) gas.…
Q: Match the confirmation test to the category of bacteria. (each category can be used multiple times)…
A: Gram-positive bacteria are bacteria that test positive in the Gram stain test, which has…
Q: 3. Based on his findings at the farm, the USDA inspector was also conçerned that the milk could be…
A: The E. coli O157:H7 is the bacterial species responsible for causing disease which typically…
Q: Differentiate between Gram positive and Gram negative cells in smears. 2. Discuss how to properly…
A: The Gram stain is the most important and commonly used staining technique in microbiology. It is…
Q: Staining microorganisms provides a wealth of information about them. A. As a laboratory technician,…
A: Gram staining technique is one of the most common techniques used to distinguish and classify…
Q: 4. Use the words presented in below to create a concept map illustrating the relationships among…
A: All these terms mentioned in the question, are related to the isolation, cultivation, and…
Q: 0.22 micrometers
A: The size of bacteria generarally ranges from 0.2 to 5 micrometer, therefore, most of the bacteria…
Q: Why is it necessary to let bacterial smears completely air dry before heat fixing?
A: To view a bacterial cell through a light microscope, its smear must be attached to a slide and then…
Q: 3. which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…
A: A dichotomous key is a toll designed by scientist, so that it can be used to identify objects and…
Q: 1. Illustrate in a diagram the identification scheme from gram-positive cocci.
A: Note: We will answer the first question since the exact one was not specified. Please submit a new…
Q: Bacterial Endospores 7. List the major steps, in sequence, needed to perform the…
A: Bacterial endospores:When there are normal environmental conditions and nutrient availability…
Q: 1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or…
A: 1. A soil-inoculated broth cannot be considered as a “pure culture”. A pure culture is a collection…
Q: Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
A: Methyl Red (MR) and Voges-Proskauer (VP) broth is used as the medium in which both the Methyl Red…
Q: 1. What kinds of media would be used to culture and Identify this microbe? 2. What are some other…
A: Hi, Thanks For Your Question. 1. What kinds of media would be used to culture and identify this…
Q: The colonies on a positive MSA plate would appear ________________. 2. Which of the following tests…
A: Introduction Coliform is the gram-negative bacterial specie that can be motile or non-motile and are…
Q: 1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to…
A: Serial dilutions of culture are needed in order to get the number of colonies on the plate within a…
Q: Explanation of results in context of unknown Test Purpose Reagents Used Gram-stain Endospore…
A: Pseudomonas aeruginosa It is an encapsulated, rod shaped bacteria which is responsible for the…
Q: 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ?
A: Answer: THERE ARE THREE TYPES OF CULTURE METHODS 1. POUR PLATE TECHNIQUE 2. STREAK PLATE TECHNIQUE…
Q: 2. Is the test tube a A) broth, B) slant, or C) deep agar medium? What is the name of the medium…
A: 1. Broth 2. Hektoen enteric agar is selective for Gram-negative bacteria.
Q: 1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to…
A: A bacteria culture is described as a type of test that is used to confirm whether you have a…
Q: 1. What is the significance of soaking the container in bleach? 2. What is the significance of…
A: Significance of container soaking in bleach before pouring :-
Q: 1. Describe the three different type of hemolysis that are observed on blood agar
A: (Note: According to guidelines, we are supposed to answer only 1 question. Please repost other…
Q: Refrigeration (~4 C) is used to slow down the spoiling of food. Which classification or…
A: Based on their optimum temperature ranges, microorganisms are classified as psychrophiles,…
Q: 3. The bacterial isolated from the abscess was also used in the test shown below. (a) What is the…
A: A pus-filled, tender to touch, inflamed region of a tissue is called abscess. Bacterial infections…
Q: What can you conclude about the Gram stained specimen? View the image in greater detail. Based on…
A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…
1. What is the most common sterilization technique used in laboratories?
2. List at least 5 procedures of the aseptic technique and describe its uses.
3. Why is Gram stain one of the most important and widely used stains in bacteriology?
4. Explain the
Step by step
Solved in 2 steps
- 1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
- 1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.1. Why do you disinfect your work area at the beginning of lab and then again at the end of lab? 2. If you have used proper aseptic technique to inoculate a new test tube of nutrient broth from apure culture of Bacillussubtilis, how many types of bacteria do you expect to grow in the new broth?And why?1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
- 1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank you1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent?3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)1. Differentiate between Gram positive and Gram negative cells in smears. 2. Discuss how to properly prepare a bacterial smear.
- 1.What would happen if an error occurs in a serial dilution? 2.Why does one cell not necessarily mean equal to one colony? 3. What would happen if incubation time of inoculated agar plates is too short or too long? 4.Why is viable plate count so convenient to apply when counting bacteria?1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)