What is the purpose of adding two drops of acetic acid in thin layer chromatography of plant pigment? What would be the effect of the following errors in chromatographic work? - a) The solvent level in the developing chamber is higher than the spotted sample. - b) Too much sample is applied to the paper. - c) The paper is allowed to remain in the chamber after the solvent front has reached the top of the plate.
What is the purpose of adding two drops of acetic acid in thin layer chromatography of plant pigment? What would be the effect of the following errors in chromatographic work? - a) The solvent level in the developing chamber is higher than the spotted sample. - b) Too much sample is applied to the paper. - c) The paper is allowed to remain in the chamber after the solvent front has reached the top of the plate.
Chapter88: Column Chromatography
Section: Chapter Questions
Problem 1P
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Question
What is the purpose of adding two drops of acetic acid in thin layer
chromatography of plant pigment?
What would be the effect of the following errors in chromatographic work?
- a) The solvent level in the developing chamber is higher than the spotted
sample.
- b) Too much sample is applied to the paper.
- c) The paper is allowed to remain in the chamber after the solvent front has reached the top of the plate.
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