What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish during inoculation?

Q: What gram-positive bacterium can give a positive presumptive test?

A: In microbiology, the presumptive test is used to find out the presence of gas-forming lactose…

Q: what is the procedure and materials you need to stain Staphylococcus aureus?

A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…

Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?

A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…

Q: What is the purpose of Thick smear? Purpose of Thin smear?

A: Blood smear:-Also known as peripheral blood smear. -Blood smear has many different uses ranging from…

Q: In what instances would the pour plate method be more appropriate than the spread plat method?

A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…

Q: Why does a streaking method used to inoculate plates result in isolated colonies?

A: Streaking method is used to obtain pure colonies of microbes(bacteria) from a proper single species…

Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?

A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…

Q: What are the most common methods of male and female surgical sterilization?

A: Fertilization is a natural process of producing children and offspring where the male gamete(sperm)…

Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…

A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…

Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…

A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…

Q: Which microbe requires serum components to be added to the growth medium?

A: Some microbes are nutritionally fastidious and hence needs some substances to be added to the growth…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: Describe the steps you would take to isolate, cultivate, and identify amicrobial pathogen from a…

A: The techniques used in the identification of microbes can be termed as the six "I's": inoculation,…

Q: What are the 4 types of culture?

A: Biological cultures include common laboratory means, which are involved in studying living organisms…

Q: Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions…

A: Antibiotic discs are used in microbiological studies to observe the effects of various antibiotics…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: What is the significance of heat fixing the smear?

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…

A: Microbiology is the branch of science which deals with the study of different types microorganisms…

Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…

A: Depending on their composition, media can be classified as defined and undefined media. Defined…

Q: Describe the manner of inoculation for different types of tube media.

A: Microbiology is the study of microorganisms and associated ideas. Microbiology has gone a long way…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: What is the importance of using a test/control organism in describing the cultural characteristics…

A: The food materials on which the organism is developed is called culture medium and the development…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: Is there a relationship between Gram staining and sensitivity to various antibacterial compounds?…

A: Gram staining is a method to identify whether a bacteria is gram positive or gram negative based on…

Q: What are some advantages and disadvantages of using blood agar vs. the Staph-Tex test to…

A: Hi, Thanks For Your Question.  Answer : Advantages Of Using Blood Agar To Differentiate Between S.…

Q: How does sterilizer spray work from drones?

A: Sterilization is a process by which a material is completely devoid of microorganisms like bacteria,…

Q: why doe the incubation time of harada mori method requires up to 10 days?

A: Introduction:- Humans are usually infected with S. stercoralis by infective filariform larvae…

Q: How common is the flotation technique being done in the Philippines?

A: Keeping in the eye the increase in a number of gastrointestinal infections, the best solution that…

Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?

A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…

Q: Before using the API® Staph System, what tests should be performed on bacteria to confirm that they…

A: API Staph (analytical profile index) is a systematized procedure used for the identification of the…

Q: What other fields of science besides microbiology is the aseptic technique used?

A: Aseptic technique refers to the procedures that prevent the contamination of microorganisms. They…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…

Q: What is the motivation behind the use of stab slant and surface inoculation

A: Agar slants are prepared by adding the agar media to the test tube and allowing it to cool in a…

Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…

A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…

Q: what is your initial assumption of the organism causing this disease

A: Bacteria are the prokaryotic microorganism and based on the structural cell walls, Gram stain…

Q: How is the Gram stain used clinically to help identify the cause of an infection?

A: Gram stain or sometimes "Gram staining", likewise called Gram's method, is a technique for staining…

Q: selective agar media for gram-positive bacteria?

A: Phenyl Ethyl Alcohol Blood Agar is a selective media for gram positive bacteria.

Q: Why is it so important to develop tests to ID bacteria

A: Answer: BACTERIA : These are the unicellular microorganisms, which are prokaryotic in nature.…

Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…

A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: In the entrropluri tube system which test require the addition of reagents after the incubation time…

A: Entreropluri test system is a Enterobacteriaceae identifying system. It is use to identify some gram…

Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…

A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…

Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…

A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.