What is the purpose of the targeting vector?

Q: name  the six different types of vectors?

A: Vector is DNA molecule that is used as a vehicle to artificially carry the gene of interest using…

Q: How can we be sure that the insertion of the therapeutic gene does not harm some other necessary…

A: The Food and Drug Administration still has not approved any human gene therapy product. Current gene…

Q: Which vector is used in the manufacturing of Covishield?

A: The delivery of vaccines require the presence of vector so that the virus can be transferred to the…

Q: What is the main problem with retroviral vectors?

A: Retroviral vectors are created by removing replication and disease-causing genes from the virus and…

Q: Why is a host-cell tRNA needed for reverse transcription?

A: Reverse transcription is a process used by retroviruses(RNA viruses) which encodes its RNA genome…

Q: How is the fragment cut and annealed to the vector?

A: Vector is the organism whose DNA is being used to carry the part of DNA from another organism. In…

Q: What is a transducing phage?

A: Transduction is the process by which foreign DNA is introduced into the cell by a suitable virus or…

Q: Who discovered the plasmids?

A: Plasmid is an extrachromosomal small DNA molecule present in a cell. Plasmid exists separately from…

Q: What are the basic steps involved in cloning foreign DNA into a vector?  How do you differentiate an…

A: Cloning Cloning refers to the making of identical copies. DNA cloning is a molecular biology…

Q: What is the difference between generalized and specialized transduction quizlet?

A:

Q: What is generalized transduction?

A: Reproduction involves the transfer of DNA from parents to offspring. Experiment of the Griffith in…

Q: What does the following vector produce? Be specific.

A: Vector is defined as a DNA molecule that is used as a vehicle to carry foreign genetic material into…

Q: Explain gene-knockout technique.

A: According to the question, we have to provide an explanation of the gene knockout technique.  So,…

Q: Name several types of vectors, and list the types of genes that they can contain.

A: The vector is a DNA molecule that serves as the carrier for the transfer or insertion of foreign…

Q: What is the receptor for the targeting sequence?

A: Proteins synthesized by ribosomes include soluble and membrane proteins of the Endoplasmic Reticulum…

Q: How has the CRISPR editing technology been appliedto targeting virus-infected eukaryotic cells?

A: The short form CRISPR refers to the Clustered Regularly Interspaced Short Palindromic Sequences.…

Q: From where Probes are derived ?

A: DNA(deoxyribonucleic acid) is a double-stranded helical genetic material containing thousands of…

Q: What is the difference between a l insertion vector such as lgt11 and a l replacement vector?

A: Vector in molecular biology is a DNA molecule which carries foreign genetic material into foreign…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: What is the difference between generalized and specialized transduction?

A: Transduction is a method of gene transfer in bacteria from donor to recipient using bacteriophage.…

Q: What is a retroviral modification ?

A: Retroviruses are a type of virus in the viral family called Retroviridae. They use RNA as their…

Q: Why is it necessary to boil the probe before you use it in your southern blot?

A: Southern blotting is a molecular biology technique that is used to detect a person's DNA. This…

Q: Define how DNA can be inserted into vectors ?

A:

Q: How to determine the location of restriction sites for different enzyme ?

A: Enzymes are proteins that are present in the body and help to speed up metabolism or other chemical…

Q: The probe in the figure below can be considered as : *

A: A biomedical device is an instrument that is used in medicine for the purpose of diagnosis,…

Q: What are the main procedures involved in a western blot?

A: BASIC INFORMATION WESTERN BLOT This process is used to find some specific type of proteins which are…

Q: What kind of vector is this?

A: This is the picture of human flea , Pulex irritans.  It is very poor vector.  It cause trench…

Q: What are the Methods of therapeutic gene delivery?

A: Gene therapy is a promising method for correcting host pathology by the manipulation of DNA…

Q: Name several types of vectors, and list the types of genes they needto function.

A: In molecular cloning, the vector is a Deoxyribose Nucleic Acid (DNA) particle that fills in as the…

Q: What is mRNA surveillance ?

A: Replication refers to a process by more copies of DNA are formed on a template DNA. Synthesis of RNA…

Q: Where did western blot get its name?

A: The western blot (also known as the protein immunoblot) or western blotting is molecular biology and…

Q: What is antiterminator hairpin ?

A: It is a secondary structure of RNA. It determines interaction in ribozyme and can help in RNA…

Q: What is the purpose of including a tube in the phage assay in which only the E. coli culture is…

A: Insects that feed on fecal, dung material and raw sewage are termed as coprophagous insects. These…

Q: What role did restriction endonucleases play in localizing the gene associated with cystic fibrosis?

A: The gene responsible for cystic fibrosis was isolated from restriction endonuclease digests.

Q: What is Northern blot analysis ?

A: The nucleic acids transfer on a solid phase membrane for identification is called the blotting…

Q: What is the nature of the targeting sequence, and what distinguishes it from other types of…

A: Introduction  In the cell, there is a highly regularized transport mechanism that works for the…

Q: What are conditional knockout mice?

A: A knockout mouse (conventional knockout) is a genetically modified mouse in which the researchers…

Q: Which vector is used to replace the defective gene in gene therapy?

A: Gene therapy focuses on utilizing the nucleic acids as a drug to treat cancer and other genetic…

Q: What is the Ti plasmid and how is it modified for the genetic modification of plants?

A: Plasmids are circular and extrachromosomal DNA (sometimes RNA) present in prokaryotic as well as…

Q: Do the plasmids integrate into the host genome when the AAV is introduced into the patient? Explain.

A: AAV (Adeno-associated viruses) are virus that are usually very small in size and that infect primate…

Q: What is gene targeting? Give some examples of gene targeting?

A: Gene is a stretch of DNA in a chromosome  which codes for a functional product either in the form of…

Q: Why is the DNA stored cold with the InstaGene matrix after boiling the samples?

A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…

Q: How is a specific probe obtained?

A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…

Q: What is the difference between the total and faecal coliform

A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…

Q: What is probe? Why it is used in the library screening ?

A: Biotechnology is the practice of using living organisms such as animals, plants, microbes, and…

Q: What is gene targeting?

A: A gene is the basic functional and physical unit of heredity. Genes are made of substance called…

Q: What must a vector contain?

A: Vector is a nucleic acid molecule containing Viral DNA or RNA and plasmid yeast or bacterial…

Q: What are PRRs, what do they do and where are they found?

A: The immune system of the human body consists of white blood cells, antibodies, spleen, thymus, and…

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.