What is trancription factor/DNA regulatory protein? What is the function of its in human body?

Q: Is lambda DNA circular or linear?

A: Lambda DNA is derived from an E.coli bacteriophage.

Q: ou are given a segment of DNA : 5’  - CATGTCAAC – 3’    What is the complimentary strand?

A: Adenine in DNA always pairs with Thymine and cytosine always pairs with Guanine and vice versa .…

Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase

A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…

Q: Do most cells contain complete copies of an organism’s DNA? Do most cells express all of the genes…

A: Genome is composed of all the genes present in an organism present in the nucleus. A genome can be…

Q: Why is only one strand transcribed, and is the same strand of DNAalways transcribed?

A: DNA (Deoxyribo Nucleic Acid) is the genetic material in all the prokaryotes are eukaryotes. DNA is…

Q: Describe the basic structural features of DNA-binding proteins that allow them to recognize specific…

A: DNA binding proteins are proteins that have DNA binding domains and thus have a specific or affinity…

Q: What is DNase hypersensitive site?

A: The DNase hypersensitive site can be recognized by the southern blotting after the limited…

Q: (b) How many forms can naturally occurring DNA exist in? Explain how these forms are characterized.…

A: DNA  Deoxyribonucleic acid  Genetic material in many species Has ds(double stranded) structure…

Q: For insertion elements and simple transposons, what is the functionof the inverted repeat sequences…

A: The sequence of DNA (deoxyribonucleic acid) that changes the position in the genome, and at times…

Q: Transposons are useful as mutagens because they act asmolecular tags for genomic DNA sequences that…

A: Step 1 Transposons or jumping gene is a DNA (deoxyribonucleic acid) sequence that can change its…

Q: What would happen if any of the stages involved in the translation of DNA to protein were…

A: Translation is a process in which ribosomes synthesizes the proteins in Cytoplasm after the…

Q: What does a deoxy nucleotide do when it gets incorporated any newly synthesize DNA and why? Where do…

A: DNA or deoxyribonucleic acid is the genetic material of most living organisms except for certain…

Q: How can these designed nucleases alter the sequence of the genomic DNA?

A: The Common approach to understand gene function in a systematic and unbiased way is including…

Q: If DNA polymerase requires a perfectly pairedprimer in order to add the next nucleotide, how is it…

A: DNA polymerases are enzymes that manufacture DNA molecules by assembling nucleotides, which are the…

Q: What are transposable or mobile DNA elements?

A: According to the question, we have to give the information on transposable or mobile DNA elements.…

Q: explain the laws of DNA polymerase? Why are they important and are all the laws required for DNA…

A: DNA polymerase is the enzyme that is helpful in catalysing the DNA molecules synthesis. There are…

Q: Why are most DNA-binding proteins specific to certain chemicalgroups within the DNA?

A: DNA is the genetic material that carries genetic information in the form of coded nucleotide…

Q: Some bacteria possess antibiotic resistance, as well as the ability to survive through adverse…

A: Bacteria  They are small prokaryotic organisms with  cell wall except some. Bacteria are both useful…

Q: Explain the cis-acting short DNA sequence elements ?

A: Gene is the basic unit of heredity of all living organisms. It is located in the nucleus organelle…

Q: What would happen to DNA repair in bacteria if MutH is mutated such that is cannot recognize the…

A: In prokaryotes , Mut H involved in mismatch repair syatem.  Mut S can recognise mismatched…

Q: Where would you expect DNA-binding proteins to bind if they recognize a specific base sequence? What…

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that transfers genetic information from one…

Q: How does DNA polymerase prevent mutations

A:

Q: eant by non-classical DNA-dependent DNA polymerases? What roles do they play in our cells?

A: DNA polymerases are enzymes that assemble nucleotides, DNA's building components, to form DNA…

Q: What are the common features of all DNA polymerases?

A: A DNA polymerase is an enzyme that catalyze the synthesis of DNA molecules from nucleoside…

Q: What would be the likely effect of a mutation that prevents σ from dissociating from the RNA…

A: After the initiation of the transcription, the RNA strand would elongate by the action of RNA…

Q: How do these engineered nucleases effect a change in the genomic DNA sequence?

A: Genome editing is a technique used to precisely and efficiently modify DNA within a cell. It…

Q: Approximately what portion of the human genome is composed of repeat sequences?

A: According to the results found in the human genome studies it is seen that the human genome has…

Q: Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the…

A: Processivity is the average number of bases a polymerase enzyme will extend and incorporate base…

Q: Why do DNA chips often contain segments derived from cDNA rather than genomic DNA segments?

A: DNA chip used in microarray

Q: What are the frequency and percentage distribution of amino acids in the polypeptides coded by the…

A: The frequency and percentage distribution of amino acids in the polypeptides coded by the Original…

Q: Which special set of enzymes in bacteria can address concerns on supercoiling when the DNA performs…

A: During replication and translation, when there is unwinding of the DNA helix, it introduces…

Q: How do P-elements move around the genome and what is the connection between transposition of mobile…

A: Genome is considered to be on the amount of DNA that is considered to be contained in a particular…

Q: How many subunits does the E. coli DNA polymerase I have? Soloct onot

A: Option(d) 1

Q: What is the architecture of the DNA polymerases and the replisome?

A: Gene is the unit of heredity. Living organisms contain genetic materials mainly made up of nucleic…

Q: What is the function of histones in DNA packiging?

A: The DNA (deoxyribonucleic acid) is a two-fold helical structure with two polynucleotide strands…

Q: Identify two major functions of DNA pol III in DNAreplication.

A: DNA replication is the natural cycle of producing two indistinguishable replicas of DNA from one…

Q: TACAT

A: Solution :There are three types of DNA Mutations: base substitutions, deletions and insertions.

Q: Which of the following is FALSE about "classic" transposons and retrotransposons? Ac is an…

A: Barbara McClintock discovered two classes of controlling elements in maize plant known as…

Q: What features distinguish a transposon from a retrotransposon?How are their sequences different, and…

A: The transposons and retrotransposons are the deoxyribonucleic acid (DNA) sequences that transfer…

Q: How many unique primers are required to amplify a single DNA target region?   a) 1 b) 2 c) 4 d)…

A: Targeted sequencing is a fast and low-cost method to detect known and novel variants in specific…

Q: How does the promoter region change from a DNase resistant site to a DNase hypersensitive site?

A: Answer: Introduction: DNase I hypersensitive sites (DHSs) are sites on chromatin which are sensitive…

Q: What will be the results of chemically modifying one nucleotide baseof a gene? What role is played…

A: A permanent change in the DNA’s base sequence is termed as mutation. Any alteration in DNA may cause…

Q: Describe how helicase, topoisomerase, and single-strand binding protein are important for the…

A: DNA replication is the process of copying the DNA of the cell. The copying of the DNA must be…

Q: How many codons are there in the mutated DNA-(b) and DNA-(c)? What types of mutations occurred in…

A: Codon is sequences of three DNA or RNA nucleotides.

Q: A mutation in which of these proteins will lead to increased mutations in all daughter cells? A.…

A: The mutation is a heritable change that arises due to the alteration of the genomic sequence of an…

Q: Describe the three important functions of DnaA protein.

A: The protein that helps in the activation of DNA replication initiation in bacteria is DnaA. It…

Q: What are the requirements for in vitro synthesis of DNA under thedirection of DNA polymerase I?

A: The formation of deoxyribonucleic acid molecules, either naturally or artificially, is known as DNA…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.